tetano
Editor, Senior Moderator
Clinical Infectious Diseases
Viral Shedding Duration of Pandemic Influenza A H1N1 Virus during an Elementary School Outbreak?Pennsylvania, May?June 2009
1. Achuyt Bhattarai1,
2. Julie Villanueva1,2,
3. Rakhee S. Palekar1,
4. Ryan Fagan1,
5. Wendy Sessions1,
6. J?rn Winter1,
7. LaShondra Berman1,
8. James Lute3,
9. Rebecca Leap1,
10. Tiffany Marchbanks3,
11. Samir V. Sodha1,
12. M?ria Moll3,
13. Xiyan Xu1,
14. Alicia Fry1,
15. Anthony Fiore1,
16. Stephen Ostroff3,
17. David L. Swerdlow1, and
18. and the Pennsylvania Working Group*
+ Author Affiliations
1.
1Centers for Disease Control and Prevention, Atlanta, Georgia
2.
2Battelle, Atlanta, Georgia
3.
3Pennsylvania Department of Health, Harrisburg, Pennsylvania
1. Correspondence: Achuyt Bhattarai, M.D., MSc, US Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop A38, Atlanta, GA 30333 (abhattarai@cdc.gov).
Abstract
We report shedding duration of 2009 pandemic influenza A (pH1N1) virus from a school-associated outbreak in Pennsylvania during May through June 2009. Outbreak-associated students or household contacts with influenza-like illness (ILI) onset within 7 days of interview were recruited. Nasopharyngeal specimens, collected every 48 hours until 2 consecutive nonpositive tests, underwent real-time reverse transcriptase polymerase chain reaction (rRT-PCR) and culture for pH1N1 virus. Culture-positive specimens underwent virus titrations. Twenty-six (median age, 8 years) rRT-PCR?positive persons, for pH1N1 virus, were included in analysis. Median shedding duration from fever onset by rRT-PCR was 6 days (range, 1?13) and 5 days (range, 1?7) by culture. Following fever resolution virus was isolated for a median of 2 days (range, 0?5). Highest and lowest virus titers detected, 2 and 5 days following fever onset, were 3.2 and 1.2 log10 TCID50/mL respectively. Overall, shedding duration in children and adults were similar to seasonal influenza viruses.
http://cid.oxfordjournals.org/content/52/suppl_1/S102.full
Viral Shedding Duration of Pandemic Influenza A H1N1 Virus during an Elementary School Outbreak?Pennsylvania, May?June 2009
1. Achuyt Bhattarai1,
2. Julie Villanueva1,2,
3. Rakhee S. Palekar1,
4. Ryan Fagan1,
5. Wendy Sessions1,
6. J?rn Winter1,
7. LaShondra Berman1,
8. James Lute3,
9. Rebecca Leap1,
10. Tiffany Marchbanks3,
11. Samir V. Sodha1,
12. M?ria Moll3,
13. Xiyan Xu1,
14. Alicia Fry1,
15. Anthony Fiore1,
16. Stephen Ostroff3,
17. David L. Swerdlow1, and
18. and the Pennsylvania Working Group*
+ Author Affiliations
1.
1Centers for Disease Control and Prevention, Atlanta, Georgia
2.
2Battelle, Atlanta, Georgia
3.
3Pennsylvania Department of Health, Harrisburg, Pennsylvania
1. Correspondence: Achuyt Bhattarai, M.D., MSc, US Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop A38, Atlanta, GA 30333 (abhattarai@cdc.gov).
Abstract
We report shedding duration of 2009 pandemic influenza A (pH1N1) virus from a school-associated outbreak in Pennsylvania during May through June 2009. Outbreak-associated students or household contacts with influenza-like illness (ILI) onset within 7 days of interview were recruited. Nasopharyngeal specimens, collected every 48 hours until 2 consecutive nonpositive tests, underwent real-time reverse transcriptase polymerase chain reaction (rRT-PCR) and culture for pH1N1 virus. Culture-positive specimens underwent virus titrations. Twenty-six (median age, 8 years) rRT-PCR?positive persons, for pH1N1 virus, were included in analysis. Median shedding duration from fever onset by rRT-PCR was 6 days (range, 1?13) and 5 days (range, 1?7) by culture. Following fever resolution virus was isolated for a median of 2 days (range, 0?5). Highest and lowest virus titers detected, 2 and 5 days following fever onset, were 3.2 and 1.2 log10 TCID50/mL respectively. Overall, shedding duration in children and adults were similar to seasonal influenza viruses.
http://cid.oxfordjournals.org/content/52/suppl_1/S102.full