tetano
Editor, Senior Moderator
Hum Vaccin Immunother. 2017 Mar 8:0. doi: 10.1080/21645515.2017.1287641. [Epub ahead of print]
[h=1]Viral Interference and the Live-attenuated Intranasal Influenza Vaccine: Results from a Pediatric Cohort with Cystic Fibrosis.[/h] Boikos C[SUP]1[/SUP], Papenburg J[SUP]2[/SUP], Martineau C[SUP]3[/SUP], Joseph L[SUP]1[/SUP], Scheifele D[SUP]4[/SUP], Chilvers M[SUP]5[/SUP], Lands LC[SUP]6[/SUP], De Serres G[SUP]7[/SUP], Quach C[SUP]1,[/SUP][SUP]2,[/SUP][SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The objective of this study was to explore the effects of viral co-detection in individuals recently vaccinated with the live-attenuated intranasal influenza virus vaccine (LAIV) on the detection of influenza RNA.
[h=4]METHODS:[/h] Before the 2013-2014 influenza season, nasal swabs were obtained from 59 pediatric participants with cystic fibrosis (CF) and 17 of their healthy siblings immediately before vaccination and 4 times during the week of follow-up. Real-time RT-PCR assays were used to detect influenza RNA. Co-detection of a non-influenza respiratory virus (NIRV) at the time of vaccination was determined by a multiplex RT-PCR assay. Differences in the proportions and rates of influenza detection and their 95% credible intervals (CrI) were estimated.
[h=4]RESULTS:[/h] Influenza RNA was detected in 16% fewer participants (95% CrI: -7, 39%) throughout follow-up in the NIRV-positive group compared to the NIRV-negative group (59% vs. 75%). This was also observed in participants with CF alone (66% vs. 74%; RD = 8% 95% CrI: -16, 33%) as well as in healthy participants only (75% vs. 30%; RD = 45%, 95% CrI: -2, 81%). Influenza was detected in NIRV-negative subjects for 0.49 days more compared to NIRV-positive subjects (95% CrI: -0.37, 1.26).
[h=4]CONCLUSION:[/h] The observed proportion of subjects in whom influenza RNA was detected and the duration of detection differed slightly between NIRV- positive and -negative subjects. However, wide credible intervals for the difference preclude definitive conclusions. If true, this observed association may be related to a recent viral respiratory infection, a phenomenon known as viral interference.
[h=4]KEYWORDS:[/h] childhood vaccination; cystic fibrosis; influenza vaccination; live-attenuated influenza virus vaccine; viral interference
PMID: 28273006 DOI: 10.1080/21645515.2017.1287641
[h=1]Viral Interference and the Live-attenuated Intranasal Influenza Vaccine: Results from a Pediatric Cohort with Cystic Fibrosis.[/h] Boikos C[SUP]1[/SUP], Papenburg J[SUP]2[/SUP], Martineau C[SUP]3[/SUP], Joseph L[SUP]1[/SUP], Scheifele D[SUP]4[/SUP], Chilvers M[SUP]5[/SUP], Lands LC[SUP]6[/SUP], De Serres G[SUP]7[/SUP], Quach C[SUP]1,[/SUP][SUP]2,[/SUP][SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The objective of this study was to explore the effects of viral co-detection in individuals recently vaccinated with the live-attenuated intranasal influenza virus vaccine (LAIV) on the detection of influenza RNA.
[h=4]METHODS:[/h] Before the 2013-2014 influenza season, nasal swabs were obtained from 59 pediatric participants with cystic fibrosis (CF) and 17 of their healthy siblings immediately before vaccination and 4 times during the week of follow-up. Real-time RT-PCR assays were used to detect influenza RNA. Co-detection of a non-influenza respiratory virus (NIRV) at the time of vaccination was determined by a multiplex RT-PCR assay. Differences in the proportions and rates of influenza detection and their 95% credible intervals (CrI) were estimated.
[h=4]RESULTS:[/h] Influenza RNA was detected in 16% fewer participants (95% CrI: -7, 39%) throughout follow-up in the NIRV-positive group compared to the NIRV-negative group (59% vs. 75%). This was also observed in participants with CF alone (66% vs. 74%; RD = 8% 95% CrI: -16, 33%) as well as in healthy participants only (75% vs. 30%; RD = 45%, 95% CrI: -2, 81%). Influenza was detected in NIRV-negative subjects for 0.49 days more compared to NIRV-positive subjects (95% CrI: -0.37, 1.26).
[h=4]CONCLUSION:[/h] The observed proportion of subjects in whom influenza RNA was detected and the duration of detection differed slightly between NIRV- positive and -negative subjects. However, wide credible intervals for the difference preclude definitive conclusions. If true, this observed association may be related to a recent viral respiratory infection, a phenomenon known as viral interference.
[h=4]KEYWORDS:[/h] childhood vaccination; cystic fibrosis; influenza vaccination; live-attenuated influenza virus vaccine; viral interference
PMID: 28273006 DOI: 10.1080/21645515.2017.1287641