tetano
Editor, Senior Moderator
Vet Q
. 2023 Jan 3;1-17.
doi: 10.1080/01652176.2023.2164909. Online ahead of print.
Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers
Juan Mena[SUP] 1 [/SUP], Christian Hidalgo[SUP] 2 [/SUP], Daniela Estay-Olea[SUP] 1 [/SUP], Nicole Sallaberry-Pincheira[SUP] 3 [/SUP], Antonella Bacigalupo[SUP] 4 [/SUP], André V Rubio[SUP] 5 [/SUP], Diego Peñaloza[SUP] 6 [/SUP], Carolina Sánchez[SUP] 3 [/SUP], Javiera Gómez-Adaros[SUP] 7 [/SUP], Valeria Olmos[SUP] 7 [/SUP], Javier Cabello[SUP] 8 [/SUP], Kendra Ivelic[SUP] 9 [/SUP], María José Abarca[SUP] 10 [/SUP], Diego Ramírez-Álvarez[SUP] 11 [/SUP], Marisol Torregrosa Rocabado[SUP] 12 [/SUP], Natalia Durán Castro[SUP] 13 [/SUP], Martina Carreño[SUP] 14 [/SUP], Gabriela Gómez[SUP] 15 [/SUP], Pedro E Cattan[SUP] 5 [/SUP], Galia Ramírez-Toloza[SUP] 16 [/SUP], Sofía Robbiano[SUP] 17 [/SUP], Carla Marchese[SUP] 18 [/SUP], Eduardo Raffo[SUP] 18 [/SUP], Paulina Stowhas[SUP] 19 [/SUP], Gonzalo Medina-Vogel[SUP] 20 [/SUP], Carlos Landaeta-Aqueveque[SUP] 21 [/SUP], René Ortega[SUP] 21 [/SUP], Etienne Waleckx[SUP] 22 23 [/SUP], Daniel Gónzalez-Acuña[SUP] 24 [/SUP], Gemma Rojo[SUP] 1 [/SUP]
Affiliations
Abstract
Background: The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals.
Aim: Molecular surveillance in different wildlife rehabilitation centers and wildlife associated institutions in Chile, which are critical points of animal-human interaction and wildlife conservation, especially since the aim of wildlife rehabilitation centers is to reintroduce animals to their original habitat.
Materials and methods: The survey was conducted in six WRCs and three wildlife associated institutions. A total of 185 samples were obtained from 83 individuals belonging to 15 different species, including vulnerable and endangered species. Each specimen was sampled with two different swabs: one oropharyngeal or nasopharyngeal according to the nostril diameter, and/or a second rectal sample. RNA was extracted from the samples and two different molecular assays were performed: first, a conventional RT-PCR with pan-coronavirus primers and a second SARS-CoV-2 qPCR targeting the N and S genes.
Results: All 185 samples were negative for SARS-CoV-2.
Keywords: COVID-19; Chile; SARS-CoV-2; wildlife conservation; wildlife rehabilitation centers
. 2023 Jan 3;1-17.
doi: 10.1080/01652176.2023.2164909. Online ahead of print.
Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers
Juan Mena[SUP] 1 [/SUP], Christian Hidalgo[SUP] 2 [/SUP], Daniela Estay-Olea[SUP] 1 [/SUP], Nicole Sallaberry-Pincheira[SUP] 3 [/SUP], Antonella Bacigalupo[SUP] 4 [/SUP], André V Rubio[SUP] 5 [/SUP], Diego Peñaloza[SUP] 6 [/SUP], Carolina Sánchez[SUP] 3 [/SUP], Javiera Gómez-Adaros[SUP] 7 [/SUP], Valeria Olmos[SUP] 7 [/SUP], Javier Cabello[SUP] 8 [/SUP], Kendra Ivelic[SUP] 9 [/SUP], María José Abarca[SUP] 10 [/SUP], Diego Ramírez-Álvarez[SUP] 11 [/SUP], Marisol Torregrosa Rocabado[SUP] 12 [/SUP], Natalia Durán Castro[SUP] 13 [/SUP], Martina Carreño[SUP] 14 [/SUP], Gabriela Gómez[SUP] 15 [/SUP], Pedro E Cattan[SUP] 5 [/SUP], Galia Ramírez-Toloza[SUP] 16 [/SUP], Sofía Robbiano[SUP] 17 [/SUP], Carla Marchese[SUP] 18 [/SUP], Eduardo Raffo[SUP] 18 [/SUP], Paulina Stowhas[SUP] 19 [/SUP], Gonzalo Medina-Vogel[SUP] 20 [/SUP], Carlos Landaeta-Aqueveque[SUP] 21 [/SUP], René Ortega[SUP] 21 [/SUP], Etienne Waleckx[SUP] 22 23 [/SUP], Daniel Gónzalez-Acuña[SUP] 24 [/SUP], Gemma Rojo[SUP] 1 [/SUP]
Affiliations
- PMID: 36594266
- DOI: 10.1080/01652176.2023.2164909
Abstract
Background: The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals.
Aim: Molecular surveillance in different wildlife rehabilitation centers and wildlife associated institutions in Chile, which are critical points of animal-human interaction and wildlife conservation, especially since the aim of wildlife rehabilitation centers is to reintroduce animals to their original habitat.
Materials and methods: The survey was conducted in six WRCs and three wildlife associated institutions. A total of 185 samples were obtained from 83 individuals belonging to 15 different species, including vulnerable and endangered species. Each specimen was sampled with two different swabs: one oropharyngeal or nasopharyngeal according to the nostril diameter, and/or a second rectal sample. RNA was extracted from the samples and two different molecular assays were performed: first, a conventional RT-PCR with pan-coronavirus primers and a second SARS-CoV-2 qPCR targeting the N and S genes.
Results: All 185 samples were negative for SARS-CoV-2.
Keywords: COVID-19; Chile; SARS-CoV-2; wildlife conservation; wildlife rehabilitation centers