tetano
Editor, Senior Moderator
Vet Microbiol
. 2025 Jan 21:302:110401.
doi: 10.1016/j.vetmic.2025.110401. Online ahead of print. Recombinant probiotic Escherichia coli delivers the polymeric protein of swine influenza virus for protection
Jin-Mei Sun[SUP] 1 [/SUP], Wen-Qiang Zhang[SUP] 1 [/SUP], Yan-Jin Li[SUP] 1 [/SUP], Tian-Kui Guo[SUP] 1 [/SUP], Rong-Rong Zhang[SUP] 1 [/SUP], Yong-Lei Yang[SUP] 1 [/SUP], Ying Zhao[SUP] 1 [/SUP], Ling-Jiao Yu[SUP] 1 [/SUP], Chun-Wei Shi[SUP] 1 [/SUP], Gui-Lian Yang[SUP] 1 [/SUP], Hai-Bin Huang[SUP] 1 [/SUP], Yan-Long Jiang[SUP] 1 [/SUP], Jian-Zhong Wang[SUP] 1 [/SUP], Xin Cao[SUP] 1 [/SUP], Nan Wang[SUP] 1 [/SUP], Yan Zeng[SUP] 1 [/SUP], Wen-Tao Yang[SUP] 2 [/SUP], Chun-Feng Wang[SUP] 3 [/SUP]
Affiliations
Swine influenza virus invades the host through the respiratory mucosa, which severely restricts the development of the pig breeding industry. To construct monomeric and trimeric vaccines, we developed recombinant Escherichia coli Nissle 1917 (EcN) strains that express the receptor binding site (RBS) of the hemagglutinin (HA) antigen from H1N1 swine influenza virus. After the mucosal immunization of mice, we found that probiotics activated CD40 and CD86 in DCs and increased the levels of IL-4 and IFN-γ secretion by T cells. Furthermore, the probiotics improved the function of the mucosal immune system, increased the level of SIgA, level of IgG and number of B220[SUP]+[/SUP]IgA[SUP]+[/SUP], and activated germinal center B cells. The challenge experiment revealed that the probiotics alleviated weight loss, reduced pathological injury to the lungs, and protected the mice from virus infection. We also observed that the serum neutralizing antibodies of immunized piglets significantly increased, which reduced the shedding frequency of swine influenza virus in the nose of the piglets and reduced the pathological damage by activating the T cell immune response in infected piglets. Thus, the constructed probiotics are promising candidates for effective non-traditional swine influenza vaccines.
Keywords: Animal experiment; Mucosal vaccine; Probiotics; RBS; Swine influenza virus.
. 2025 Jan 21:302:110401.
doi: 10.1016/j.vetmic.2025.110401. Online ahead of print. Recombinant probiotic Escherichia coli delivers the polymeric protein of swine influenza virus for protection
Jin-Mei Sun[SUP] 1 [/SUP], Wen-Qiang Zhang[SUP] 1 [/SUP], Yan-Jin Li[SUP] 1 [/SUP], Tian-Kui Guo[SUP] 1 [/SUP], Rong-Rong Zhang[SUP] 1 [/SUP], Yong-Lei Yang[SUP] 1 [/SUP], Ying Zhao[SUP] 1 [/SUP], Ling-Jiao Yu[SUP] 1 [/SUP], Chun-Wei Shi[SUP] 1 [/SUP], Gui-Lian Yang[SUP] 1 [/SUP], Hai-Bin Huang[SUP] 1 [/SUP], Yan-Long Jiang[SUP] 1 [/SUP], Jian-Zhong Wang[SUP] 1 [/SUP], Xin Cao[SUP] 1 [/SUP], Nan Wang[SUP] 1 [/SUP], Yan Zeng[SUP] 1 [/SUP], Wen-Tao Yang[SUP] 2 [/SUP], Chun-Feng Wang[SUP] 3 [/SUP]
Affiliations
- PMID: 39862797
- DOI: 10.1016/j.vetmic.2025.110401
Swine influenza virus invades the host through the respiratory mucosa, which severely restricts the development of the pig breeding industry. To construct monomeric and trimeric vaccines, we developed recombinant Escherichia coli Nissle 1917 (EcN) strains that express the receptor binding site (RBS) of the hemagglutinin (HA) antigen from H1N1 swine influenza virus. After the mucosal immunization of mice, we found that probiotics activated CD40 and CD86 in DCs and increased the levels of IL-4 and IFN-γ secretion by T cells. Furthermore, the probiotics improved the function of the mucosal immune system, increased the level of SIgA, level of IgG and number of B220[SUP]+[/SUP]IgA[SUP]+[/SUP], and activated germinal center B cells. The challenge experiment revealed that the probiotics alleviated weight loss, reduced pathological injury to the lungs, and protected the mice from virus infection. We also observed that the serum neutralizing antibodies of immunized piglets significantly increased, which reduced the shedding frequency of swine influenza virus in the nose of the piglets and reduced the pathological damage by activating the T cell immune response in infected piglets. Thus, the constructed probiotics are promising candidates for effective non-traditional swine influenza vaccines.
Keywords: Animal experiment; Mucosal vaccine; Probiotics; RBS; Swine influenza virus.