tetano
Editor, Senior Moderator
Vet Microbiol
. 2026 Oct 1:322:111238.
doi: 10.1016/j.vetmic.2026.111238. Online ahead of print.
Wenjie Jiang 1 , Weikang Wang 2 , Xinran Chu 3 , Zhehong Zhao 4 , Xudong Cao 5 , Xuefeng Yin 6 , Tuofan Li 7 , Hongxia Shao 8 , Zhimin Wan 9 , Aijian Qin 10 , Quan Xie 11 , Jianqiang Ye 12
Affiliations
The recombinant H3N8 avian influenza virus (AIV) continues to circulate among poultry in China, with sporadic human infections reported, posing a significant threat to both the poultry industry and public health. However, no commercial vaccine against H3N8 AIV is currently available. Our previous studies have showed that serotype 4 fowl adenovirus (FAdV-4) with the deletion of fiber-2 gene is highly attenuated and serves as an ideal vaccine vector for expressing foreign genes. In this study, a novel recombinant FAdV-4 carrying HA of H3N8 was generated by targeting fiber-2 through the CRISPR/Cas9 and Cre-LoxP systems, designed as FA4-CMV-H3. In vitro, studies demonstrated that FA4-CMV-H3 not only efficiently expressed the HA protein of H3N8 but also replicated efficiently in LMH cells stably expressing Fiber-2 protein of FAdV-4. Chicken studies showed that FA4-CMV-H3 is highly attenuated in vivo and induces high neutralizing antibodies against FAdV-4 and high HI titers against H3N8. Moreover, FA4-CMV-H3 could provide full protection against both H3N8 and FAdV-4 challenges. All these demonstrate that the generated recombinant virus FA4-CMV-H3 is a promising bivalent efficient vaccine candidate against both FAdV-4 and H3N8.
Keywords: Attenuated vaccine; CRISPR/Cas9; Chicken; FAdV-4; H3N8; HA; Protection; Recombinant viruses.
. 2026 Oct 1:322:111238.
doi: 10.1016/j.vetmic.2026.111238. Online ahead of print.
A novel recombinant serotype 4 fowl adenovirus expressing HA of H3N8 avian influenza virus
Wenjie Jiang 1 , Weikang Wang 2 , Xinran Chu 3 , Zhehong Zhao 4 , Xudong Cao 5 , Xuefeng Yin 6 , Tuofan Li 7 , Hongxia Shao 8 , Zhimin Wan 9 , Aijian Qin 10 , Quan Xie 11 , Jianqiang Ye 12
Affiliations
- PMID: 42826490
- DOI: 10.1016/j.vetmic.2026.111238
Abstract
The recombinant H3N8 avian influenza virus (AIV) continues to circulate among poultry in China, with sporadic human infections reported, posing a significant threat to both the poultry industry and public health. However, no commercial vaccine against H3N8 AIV is currently available. Our previous studies have showed that serotype 4 fowl adenovirus (FAdV-4) with the deletion of fiber-2 gene is highly attenuated and serves as an ideal vaccine vector for expressing foreign genes. In this study, a novel recombinant FAdV-4 carrying HA of H3N8 was generated by targeting fiber-2 through the CRISPR/Cas9 and Cre-LoxP systems, designed as FA4-CMV-H3. In vitro, studies demonstrated that FA4-CMV-H3 not only efficiently expressed the HA protein of H3N8 but also replicated efficiently in LMH cells stably expressing Fiber-2 protein of FAdV-4. Chicken studies showed that FA4-CMV-H3 is highly attenuated in vivo and induces high neutralizing antibodies against FAdV-4 and high HI titers against H3N8. Moreover, FA4-CMV-H3 could provide full protection against both H3N8 and FAdV-4 challenges. All these demonstrate that the generated recombinant virus FA4-CMV-H3 is a promising bivalent efficient vaccine candidate against both FAdV-4 and H3N8.
Keywords: Attenuated vaccine; CRISPR/Cas9; Chicken; FAdV-4; H3N8; HA; Protection; Recombinant viruses.