Biological
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Ian McKay at VDU Reviews 3 articles ( here's one clip)
Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation : The Lancet Infectious Diseases (closed access)
Some key findings here include: ...by Ian McKay at VDU
One Clip:
•The article's introduction suggests that the genetic diversity of human MERS-CoV viruses determined to date is the result of multiple zoonotic acquisitions
•This study started with a 61-year-old Qatari male (61M; FT#144) farm owner who had not travelled outside Qatar and his 23-year-old male (23M; FT#150) employee
•61M was RT-PCR POS (upE assay) on a sputum sample (collected Oct-13) and 23M on a throat swab (collected Oct-17) and subgenomic (ORF1b and nucleocapsid [N]) sequencing at the Public Health England confirmed the detection to be MERS-CoV
•MERS-CoV genomic sequences from the 2 human cases were placed on GenBank and called Qatar_3_2013 [61M] and Qatar_4_2013 [23M] as were camel sequences from the subsequent experiments
•Sera, rectal swabs and flocked nasal swabs were collected from all of the farm's 14 camels as well as 5 stool samples from 3 cages, by a team wearing personal protective equipment. Samples were shipped to the Netherlands for upE, N and ORF1a RT-PCR testing
•Vero and Huh-7 cells were inoculated with swabs that had been added to viral transport medium onsite. A single culture from Camel#7 was upE RT-PCR positive at day-4 after inoculation but no culture yielded infectious virus
•5/14 camels were MERS-CoV PCR positive using upE, N and ORF1a assays
•Sequencing of a fragment of Spike gene yielded 100% identity with other Saudi MERS-CoV sequences; sequence only differing by 1 base from the original isolate, MERS-CoV/EMC.
•All camel sera were antibody positive using an immunofluorescence test on MERS-CoV/EMC-infected cells
•There was no "direction" to the acquisition of MERS-CoV. Whether the camels infected the humans or the humans infected the camels could not be determined from this outbreak
•The authors conclude that detailed cases histories are important to identify animal exposures. These might not otherwise be though important in a cursory question and answer of a patient, their family or contacts
Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation : The Lancet Infectious Diseases
http://www.thelancet.com/journals/laninf/article/PIIS1473-3099(13)70690-X/fulltext#article_upsell
see more information....
Via VDU
http://virologydownunder.blogspot.com.au/
H/T Ian McKay at VDU
Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation : The Lancet Infectious Diseases (closed access)
Some key findings here include: ...by Ian McKay at VDU
One Clip:
•The article's introduction suggests that the genetic diversity of human MERS-CoV viruses determined to date is the result of multiple zoonotic acquisitions
•This study started with a 61-year-old Qatari male (61M; FT#144) farm owner who had not travelled outside Qatar and his 23-year-old male (23M; FT#150) employee
•61M was RT-PCR POS (upE assay) on a sputum sample (collected Oct-13) and 23M on a throat swab (collected Oct-17) and subgenomic (ORF1b and nucleocapsid [N]) sequencing at the Public Health England confirmed the detection to be MERS-CoV
•MERS-CoV genomic sequences from the 2 human cases were placed on GenBank and called Qatar_3_2013 [61M] and Qatar_4_2013 [23M] as were camel sequences from the subsequent experiments
•Sera, rectal swabs and flocked nasal swabs were collected from all of the farm's 14 camels as well as 5 stool samples from 3 cages, by a team wearing personal protective equipment. Samples were shipped to the Netherlands for upE, N and ORF1a RT-PCR testing
•Vero and Huh-7 cells were inoculated with swabs that had been added to viral transport medium onsite. A single culture from Camel#7 was upE RT-PCR positive at day-4 after inoculation but no culture yielded infectious virus
•5/14 camels were MERS-CoV PCR positive using upE, N and ORF1a assays
•Sequencing of a fragment of Spike gene yielded 100% identity with other Saudi MERS-CoV sequences; sequence only differing by 1 base from the original isolate, MERS-CoV/EMC.
•All camel sera were antibody positive using an immunofluorescence test on MERS-CoV/EMC-infected cells
•There was no "direction" to the acquisition of MERS-CoV. Whether the camels infected the humans or the humans infected the camels could not be determined from this outbreak
•The authors conclude that detailed cases histories are important to identify animal exposures. These might not otherwise be though important in a cursory question and answer of a patient, their family or contacts
Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation : The Lancet Infectious Diseases
http://www.thelancet.com/journals/laninf/article/PIIS1473-3099(13)70690-X/fulltext#article_upsell
see more information....
Via VDU
http://virologydownunder.blogspot.com.au/
H/T Ian McKay at VDU