tetano
Editor, Senior Moderator
J Med Microbiol. 2019 Jul 2. doi: 10.1099/jmm.0.001032. [Epub ahead of print]
[h=1]Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.[/h] LeBlanc JJ[SUP]1[/SUP], ElSherif M[SUP]1[/SUP], Mulpuru S[SUP]2[/SUP], Warhuus M[SUP]1[/SUP], Ambrose A[SUP]1[/SUP], Andrew M[SUP]1[/SUP], Boivin G[SUP]3[/SUP], Bowie W[SUP]4[/SUP], Chit A[SUP]5,[/SUP][SUP]6[/SUP], Dos Santos G[SUP]7[/SUP], Green K[SUP]8[/SUP], Halperin SA[SUP]1[/SUP], Hatchette TF[SUP]1[/SUP], Ibarguchi B[SUP]9[/SUP], Johnstone J[SUP]10[/SUP], Katz K[SUP]11[/SUP], Langley JM[SUP]1[/SUP], Lagac?-Wiens P[SUP]12[/SUP], Loeb M[SUP]10[/SUP], Lund A[SUP]1[/SUP], MacKinnon-Cameron D[SUP]1[/SUP], McCarthy A[SUP]13[/SUP], McElhaney JE[SUP]14[/SUP], McGeer A[SUP]8[/SUP], Poirier A[SUP]15[/SUP], Powis J[SUP]16[/SUP], Richardson D[SUP]17[/SUP], Semret M[SUP]18[/SUP], Shinde V[SUP]19[/SUP], Smyth D[SUP]20[/SUP], Trottier S[SUP]3[/SUP], Valiquette L[SUP]21[/SUP], Webster D[SUP]22[/SUP], Ye L[SUP]1[/SUP], McNeil SA[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.
[h=4]METHODS:[/h] Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.
[h=4]RESULTS:[/h] In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.
[h=4]CONCLUSIONS:[/h] Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.
PMID: 31264957 DOI: 10.1099/jmm.0.001032
[h=1]Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.[/h] LeBlanc JJ[SUP]1[/SUP], ElSherif M[SUP]1[/SUP], Mulpuru S[SUP]2[/SUP], Warhuus M[SUP]1[/SUP], Ambrose A[SUP]1[/SUP], Andrew M[SUP]1[/SUP], Boivin G[SUP]3[/SUP], Bowie W[SUP]4[/SUP], Chit A[SUP]5,[/SUP][SUP]6[/SUP], Dos Santos G[SUP]7[/SUP], Green K[SUP]8[/SUP], Halperin SA[SUP]1[/SUP], Hatchette TF[SUP]1[/SUP], Ibarguchi B[SUP]9[/SUP], Johnstone J[SUP]10[/SUP], Katz K[SUP]11[/SUP], Langley JM[SUP]1[/SUP], Lagac?-Wiens P[SUP]12[/SUP], Loeb M[SUP]10[/SUP], Lund A[SUP]1[/SUP], MacKinnon-Cameron D[SUP]1[/SUP], McCarthy A[SUP]13[/SUP], McElhaney JE[SUP]14[/SUP], McGeer A[SUP]8[/SUP], Poirier A[SUP]15[/SUP], Powis J[SUP]16[/SUP], Richardson D[SUP]17[/SUP], Semret M[SUP]18[/SUP], Shinde V[SUP]19[/SUP], Smyth D[SUP]20[/SUP], Trottier S[SUP]3[/SUP], Valiquette L[SUP]21[/SUP], Webster D[SUP]22[/SUP], Ye L[SUP]1[/SUP], McNeil SA[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.
[h=4]METHODS:[/h] Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.
[h=4]RESULTS:[/h] In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.
[h=4]CONCLUSIONS:[/h] Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.
PMID: 31264957 DOI: 10.1099/jmm.0.001032