tetano
Editor, Senior Moderator
Vaccines (Basel)
. 2022 Sep 19;10(9):1559.
doi: 10.3390/vaccines10091559.
Intranasal Treatment of Ferrets with Inert Bacterial Spores Reduces Disease Caused by a Challenging H7N9 Avian Influenza Virus
Joe James[SUP] 1 [/SUP], Stephanie M Meyer[SUP] 1 [/SUP], Huynh A Hong[SUP] 2 [/SUP], Chau Dang[SUP] 2 [/SUP], Ho T Y Linh[SUP] 3 [/SUP], William Ferreira[SUP] 2 [/SUP], Paidamoyo M Katsande[SUP] 4 [/SUP], Linh Vo[SUP] 2 [/SUP], Daniel Hynes[SUP] 5 [/SUP], William Love[SUP] 5 [/SUP], Ashley C Banyard[SUP] 1 [/SUP], Simon M Cutting[SUP] 2 4 [/SUP]
Affiliations
Abstract
Background: Influenza is a respiratory infection that continues to present a major threat to human health, with ~500,000 deaths/year. Continued circulation of epidemic subtypes in humans and animals potentially increases the risk of future pandemics. Vaccination has failed to halt the evolution of this virus and next-generation prophylactic approaches are under development. Naked, "heat inactivated", or inert bacterial spores have been shown to protect against influenza in murine models. Methods: Ferrets were administered intranasal doses of inert bacterial spores (DSM 32444[SUP]K[/SUP]) every 7 days for 4 weeks. Seven days after the last dose, the animals were challenged with avian H7N9 influenza A virus. Clinical signs of infection and viral shedding were monitored. Results: Clinical symptoms of infection were significantly reduced in animals dosed with DSM 32444[SUP]K[/SUP]. The temporal kinetics of viral shedding was reduced but not prevented. Conclusion: Taken together, nasal dosing using heat-stable spores could provide a useful approach for influenza prophylaxis in both humans and animals.
Keywords: Bacillus subtilis; bacterial spores; influenza; innate immunity; prophylaxis.
. 2022 Sep 19;10(9):1559.
doi: 10.3390/vaccines10091559.
Intranasal Treatment of Ferrets with Inert Bacterial Spores Reduces Disease Caused by a Challenging H7N9 Avian Influenza Virus
Joe James[SUP] 1 [/SUP], Stephanie M Meyer[SUP] 1 [/SUP], Huynh A Hong[SUP] 2 [/SUP], Chau Dang[SUP] 2 [/SUP], Ho T Y Linh[SUP] 3 [/SUP], William Ferreira[SUP] 2 [/SUP], Paidamoyo M Katsande[SUP] 4 [/SUP], Linh Vo[SUP] 2 [/SUP], Daniel Hynes[SUP] 5 [/SUP], William Love[SUP] 5 [/SUP], Ashley C Banyard[SUP] 1 [/SUP], Simon M Cutting[SUP] 2 4 [/SUP]
Affiliations
- PMID: 36146637
- DOI: 10.3390/vaccines10091559
Abstract
Background: Influenza is a respiratory infection that continues to present a major threat to human health, with ~500,000 deaths/year. Continued circulation of epidemic subtypes in humans and animals potentially increases the risk of future pandemics. Vaccination has failed to halt the evolution of this virus and next-generation prophylactic approaches are under development. Naked, "heat inactivated", or inert bacterial spores have been shown to protect against influenza in murine models. Methods: Ferrets were administered intranasal doses of inert bacterial spores (DSM 32444[SUP]K[/SUP]) every 7 days for 4 weeks. Seven days after the last dose, the animals were challenged with avian H7N9 influenza A virus. Clinical signs of infection and viral shedding were monitored. Results: Clinical symptoms of infection were significantly reduced in animals dosed with DSM 32444[SUP]K[/SUP]. The temporal kinetics of viral shedding was reduced but not prevented. Conclusion: Taken together, nasal dosing using heat-stable spores could provide a useful approach for influenza prophylaxis in both humans and animals.
Keywords: Bacillus subtilis; bacterial spores; influenza; innate immunity; prophylaxis.