tetano
Editor, Senior Moderator
Vaccines (Basel)
. 2020 Jul 3;8(3):E355.
doi: 10.3390/vaccines8030355.
Immunoinformatic Analysis of T- And B-Cell Epitopes for SARS-CoV-2 Vaccine Design
Dongliang Wang[SUP] 1 [/SUP], Jinhui Mai[SUP] 1 [/SUP], Wenfeng Zhou[SUP] 1 [/SUP], Wanting Yu[SUP] 1 2 [/SUP], Yang Zhan[SUP] 1 [/SUP], Naidong Wang[SUP] 1 [/SUP], Neal D Epstein[SUP] 3 [/SUP], Yi Yang[SUP] 1 [/SUP]
Affiliations
Abstract
Currently, there is limited knowledge about the immunological profiles of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). We used computer-based immunoinformatic analysis and the newly resolved 3-dimensional (3D) structures of the SARS-CoV-2 S trimeric protein, together with analyses of the immunogenic profiles of SARS-CoV, to anticipate potential B-cell and T-cell epitopes of the SARS-CoV-2 S protein for vaccine design, particularly for peptide-driven vaccine design and serological diagnosis. Nine conserved linear B-cell epitopes and multiple discontinuous B-cell epitopes composed of 69 residues on the surface of the SARS-CoV-2 trimeric S protein were predicted to be highly antigenic. We found that the SARS-CoV-2 S protein has a different antigenic profile than that of the SARS-CoV S protein due to the variations in their primary and 3D structures. Importantly, SARS-CoV-2 may exploit an immune evasion mechanism through two point mutations in the critical and conserved linear neutralization epitope (overlap with fusion peptide) around a sparsely glycosylated area. These mutations lead to a significant decrease in the antigenicity of this epitope in the SARS-CoV-2 S protein. In addition, 62 T-cell epitopes in the SARS-CoV-2 S protein were predicted in our study. The structure-based immunoinformatic analysis for the SARS-CoV-2 S protein in this study may improve vaccine design, diagnosis, and immunotherapy against the pandemic of COVID-19.
Keywords: B-cell and T-cell epitopes; S protein; SARS-CoV-2; vaccine.
. 2020 Jul 3;8(3):E355.
doi: 10.3390/vaccines8030355.
Immunoinformatic Analysis of T- And B-Cell Epitopes for SARS-CoV-2 Vaccine Design
Dongliang Wang[SUP] 1 [/SUP], Jinhui Mai[SUP] 1 [/SUP], Wenfeng Zhou[SUP] 1 [/SUP], Wanting Yu[SUP] 1 2 [/SUP], Yang Zhan[SUP] 1 [/SUP], Naidong Wang[SUP] 1 [/SUP], Neal D Epstein[SUP] 3 [/SUP], Yi Yang[SUP] 1 [/SUP]
Affiliations
- PMID: 32635180
- DOI: 10.3390/vaccines8030355
Abstract
Currently, there is limited knowledge about the immunological profiles of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). We used computer-based immunoinformatic analysis and the newly resolved 3-dimensional (3D) structures of the SARS-CoV-2 S trimeric protein, together with analyses of the immunogenic profiles of SARS-CoV, to anticipate potential B-cell and T-cell epitopes of the SARS-CoV-2 S protein for vaccine design, particularly for peptide-driven vaccine design and serological diagnosis. Nine conserved linear B-cell epitopes and multiple discontinuous B-cell epitopes composed of 69 residues on the surface of the SARS-CoV-2 trimeric S protein were predicted to be highly antigenic. We found that the SARS-CoV-2 S protein has a different antigenic profile than that of the SARS-CoV S protein due to the variations in their primary and 3D structures. Importantly, SARS-CoV-2 may exploit an immune evasion mechanism through two point mutations in the critical and conserved linear neutralization epitope (overlap with fusion peptide) around a sparsely glycosylated area. These mutations lead to a significant decrease in the antigenicity of this epitope in the SARS-CoV-2 S protein. In addition, 62 T-cell epitopes in the SARS-CoV-2 S protein were predicted in our study. The structure-based immunoinformatic analysis for the SARS-CoV-2 S protein in this study may improve vaccine design, diagnosis, and immunotherapy against the pandemic of COVID-19.
Keywords: B-cell and T-cell epitopes; S protein; SARS-CoV-2; vaccine.