tetano
Editor, Senior Moderator
Vaccine
. 2022 Mar 29;S0264-410X(22)00369-3.
doi: 10.1016/j.vaccine.2022.03.057. Online ahead of print.
Vaccine-induced humoral response against SARS-CoV-2 dramatically declined but cellular immunity possibly remained at 6 months post BNT162b2 vaccination
Hideaki Kato[SUP] 1 [/SUP], Kei Miyakawa[SUP] 2 [/SUP], Norihisa Ohtake[SUP] 3 [/SUP], Yutaro Yamaoka[SUP] 4 [/SUP], Satoshi Yajima[SUP] 5 [/SUP], Etsuko Yamazaki[SUP] 5 [/SUP], Tomoko Shimada[SUP] 6 [/SUP], Atsushi Goto[SUP] 7 [/SUP], Hideaki Nakajima[SUP] 8 [/SUP], Akihide Ryo[SUP] 9 [/SUP]
Affiliations
Abstract
To evaluate vaccine-induced humoral and cell-mediated immunity at 6 months after completion of two doses of BNT162b2 vaccination, immunoglobulin G against SARS-CoV-2 spike protein (SP IgG), 50% neutralizing antibody (NT[SUB]50[/SUB]), and spot-forming cell (SFC) counts were evaluated by interferon-γ releasing ELISpot assay of 98 healthy subjects (median age, 43 years). The geometric mean titers of SP IgG and NT[SUB]50[/SUB] decreased from 95.2 (95% confidence interval (CI) 79.8-113.4) to 5.7 (95% CI 4.9-6.7) and from 680.4 (588.0-787.2) to 130.4 (95% CI 104.2-163.1), respectively, at 3 weeks and 6 months after the vaccination. SP IgG titer was negatively correlated with age and alcohol consumption. Spot-forming cell counts at 6 months did not correlate with age, gender, and other parameters of the patients. SP IgG, NT[SUB]50,[/SUB] and SFC titers were elevated in the breakthrough infected subjects. Although the levels of vaccine-induced antibodies dramatically declined at 6 months after vaccination, a certain degree of cellular immunity was observed irrespective of the age.
Keywords: 50% neutralization titer; Cell-mediated immunity; Coronavirus disease-2019; Interferon-γ releasing ELISpot assay; Vaccination; Variant of concern.
. 2022 Mar 29;S0264-410X(22)00369-3.
doi: 10.1016/j.vaccine.2022.03.057. Online ahead of print.
Vaccine-induced humoral response against SARS-CoV-2 dramatically declined but cellular immunity possibly remained at 6 months post BNT162b2 vaccination
Hideaki Kato[SUP] 1 [/SUP], Kei Miyakawa[SUP] 2 [/SUP], Norihisa Ohtake[SUP] 3 [/SUP], Yutaro Yamaoka[SUP] 4 [/SUP], Satoshi Yajima[SUP] 5 [/SUP], Etsuko Yamazaki[SUP] 5 [/SUP], Tomoko Shimada[SUP] 6 [/SUP], Atsushi Goto[SUP] 7 [/SUP], Hideaki Nakajima[SUP] 8 [/SUP], Akihide Ryo[SUP] 9 [/SUP]
Affiliations
- PMID: 35370020
- DOI: 10.1016/j.vaccine.2022.03.057
Abstract
To evaluate vaccine-induced humoral and cell-mediated immunity at 6 months after completion of two doses of BNT162b2 vaccination, immunoglobulin G against SARS-CoV-2 spike protein (SP IgG), 50% neutralizing antibody (NT[SUB]50[/SUB]), and spot-forming cell (SFC) counts were evaluated by interferon-γ releasing ELISpot assay of 98 healthy subjects (median age, 43 years). The geometric mean titers of SP IgG and NT[SUB]50[/SUB] decreased from 95.2 (95% confidence interval (CI) 79.8-113.4) to 5.7 (95% CI 4.9-6.7) and from 680.4 (588.0-787.2) to 130.4 (95% CI 104.2-163.1), respectively, at 3 weeks and 6 months after the vaccination. SP IgG titer was negatively correlated with age and alcohol consumption. Spot-forming cell counts at 6 months did not correlate with age, gender, and other parameters of the patients. SP IgG, NT[SUB]50,[/SUB] and SFC titers were elevated in the breakthrough infected subjects. Although the levels of vaccine-induced antibodies dramatically declined at 6 months after vaccination, a certain degree of cellular immunity was observed irrespective of the age.
Keywords: 50% neutralization titer; Cell-mediated immunity; Coronavirus disease-2019; Interferon-γ releasing ELISpot assay; Vaccination; Variant of concern.