Giuseppe
Emeritus
Vaccine. 2008 Dec 9;26(52):6852-8.
Timely production of A/Fujian-like influenza vaccine matching the 2003-2004 epidemic strain may have been possible using Madin-Darby canine kidney cells.
Makizumi K, Kimachi K, Fukada K, Nishimura T, Kudo Y, Goto S, Odagiri T, Tashiro M, Kino Y. - The Chemo-Sero-Therapeutic Research Institute, Kyokushi, Kikuchi, Kumamoto 869-1298, Japan. makizumi@kaketsuken.or.jp
Timely production and antigenic match with those of the epidemic strains are required for influenza vaccines.
A/Fujian/411/2002-like (H3N2) virus was the main epidemic influenza virus during the 2003/2004 season in the northern hemisphere.
But A/Fujian-like reassortant viruses were not available until more than one year later.
We evaluated the A/Kumamoto/102/2002 strain, an A/Fujian/411/2002-like strain isolated in 2002, as a potential vaccine.
We compared A/Kumamoto/102/2002 viruses isolated from the same clinical sample in Madin-Darby canine kidney (MDCK) cells and eggs.
Kumamoto/102/2002 isolated from eggs grew poorly and showed amino acid mutations of haemagglutinin.
In contrast, A/Kumamoto/102/2002 isolated from MDCK cells grew well in MDCK suspension culture.
The amino acid sequence of MDCK-derived A/Kumamoto virus was identical to that of A/Fujian/411/2002.
These results suggest that culture in MDCK cells could have produced an influenza vaccine with a better antigenetic match to the predicted epidemic strain for the 2003/2004 season than the vaccine actually produced.
PMID: 19149013 [PubMed - in process]
-
------
-
Timely production of A/Fujian-like influenza vaccine matching the 2003-2004 epidemic strain may have been possible using Madin-Darby canine kidney cells.
Makizumi K, Kimachi K, Fukada K, Nishimura T, Kudo Y, Goto S, Odagiri T, Tashiro M, Kino Y. - The Chemo-Sero-Therapeutic Research Institute, Kyokushi, Kikuchi, Kumamoto 869-1298, Japan. makizumi@kaketsuken.or.jp
Timely production and antigenic match with those of the epidemic strains are required for influenza vaccines.
A/Fujian/411/2002-like (H3N2) virus was the main epidemic influenza virus during the 2003/2004 season in the northern hemisphere.
But A/Fujian-like reassortant viruses were not available until more than one year later.
We evaluated the A/Kumamoto/102/2002 strain, an A/Fujian/411/2002-like strain isolated in 2002, as a potential vaccine.
We compared A/Kumamoto/102/2002 viruses isolated from the same clinical sample in Madin-Darby canine kidney (MDCK) cells and eggs.
Kumamoto/102/2002 isolated from eggs grew poorly and showed amino acid mutations of haemagglutinin.
In contrast, A/Kumamoto/102/2002 isolated from MDCK cells grew well in MDCK suspension culture.
The amino acid sequence of MDCK-derived A/Kumamoto virus was identical to that of A/Fujian/411/2002.
These results suggest that culture in MDCK cells could have produced an influenza vaccine with a better antigenetic match to the predicted epidemic strain for the 2003/2004 season than the vaccine actually produced.
PMID: 19149013 [PubMed - in process]
-
------
-