Giuseppe
Emeritus
Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing. (Vaccine, abstract, edited)
[Source: US National Library of Medicine, (LINK). Edited.]
Vaccine. 2010 Mar 25. [Epub ahead of print]
Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing.
Le Ru A, Jacob D, Transfiguracion J, Ansorge S, Henry O, Kamen AA. - Animal Cell Technology, National Research Council of Canada, 6100 Royalmount Avenue, Montr?al, Qu?bec, Canada H4P 2R2; Chemical Engineering Department, Ecole Polytechnique, Montr?al, Qu?bec, Canada H3T 1J4.
Cell culture processes offer an attractive alternative to conventional chicken egg-based influenza vaccine production methods. However, most protocols still rely on the use of adherent cells, which makes process scale-up a challenging issue. In this study, it is demonstrated that the HEK-293 human cell line is able to efficiently replicate influenza virus. Production in serum-free suspension of HEK-293 cultures resulted in high titers of infectious influenza viruses for different subtypes and variants including A/H1, A/H3 and B strains. After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 10(9)IVP/mL demonstrating a potential for scale-up.
Copyright ? 2010. Published by Elsevier Ltd.
PMID: 20347632 [PubMed - as supplied by publisher]
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[Source: US National Library of Medicine, (LINK). Edited.]
Vaccine. 2010 Mar 25. [Epub ahead of print]
Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing.
Le Ru A, Jacob D, Transfiguracion J, Ansorge S, Henry O, Kamen AA. - Animal Cell Technology, National Research Council of Canada, 6100 Royalmount Avenue, Montr?al, Qu?bec, Canada H4P 2R2; Chemical Engineering Department, Ecole Polytechnique, Montr?al, Qu?bec, Canada H3T 1J4.
Cell culture processes offer an attractive alternative to conventional chicken egg-based influenza vaccine production methods. However, most protocols still rely on the use of adherent cells, which makes process scale-up a challenging issue. In this study, it is demonstrated that the HEK-293 human cell line is able to efficiently replicate influenza virus. Production in serum-free suspension of HEK-293 cultures resulted in high titers of infectious influenza viruses for different subtypes and variants including A/H1, A/H3 and B strains. After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 10(9)IVP/mL demonstrating a potential for scale-up.
Copyright ? 2010. Published by Elsevier Ltd.
PMID: 20347632 [PubMed - as supplied by publisher]
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