Vaccine. Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotian

[Giuseppe]

Vaccine. 2009 Feb 4. [Epub ahead of print]

Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.

Shoji Y, Farrance CE, Bi H, Shamloul M, Green B, Manceva S, Rhee A, Ugulava N, Roy G, Musiychuk K, Chichester JA, Mett V, Yusibov V. - Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE 19711, USA.

Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype have been identified as a potential pandemic threat by the World Health Organization (WHO). Since 1997, these viruses have been spreading from Asia to Europe and Africa with increasing genetic and antigenic diversities.
Vaccination is the preferred strategy for the prevention and control of influenza infections and the availability of a system for the rapid engineering and production of vaccines is required in the event of an influenza pandemic.
In this study, we engineered and produced recombinant hemagglutinin (HA) from A/Bar-headed Goose/Qinghai/1A/05 (clade 2.2) and A/Anhui/1/2005 (clade 2.3) in Nicotiana benthamiana plants.
Immunization of mice with these plant-derived HA antigens elicited serum hemagglutination inhibition (HI) and virus neutralization (VN) antibodies. These results suggest the utility of our plant-expression system for recombinant influenza vaccine production.

PMID: 19200814 [PubMed - as supplied by publisher]
-
------

 

[gsgs]

Re: Vaccine. Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.

what's the advantage of plants over bioreactors ?

 

[Sally Furniss]

Re: Vaccine. Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.

Influenza Hemagglutinin Expression in Nicotiana tabacum and Nicotiana benthamiana

http://www.flutrackers.com/forum/showthread.php?t=94401

 

[Sally Furniss]

Re: Vaccine. Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.

Nicotiana tabacum in bioreactors



Titre du document / Document title

Bioreactor operation for transgenic Nicotiana tabacum cell cultures and continuous production of recombinant human granulocyte-macrophage colony-stimulating factor by perfusion cultureAuteur(s) / Author(s)

LEE Sang-Yoon⁽¹⁾ ; KIM Yong-Hoon⁽¹⁾ ; ROH Yun-Sook⁽¹⁾ ; MYOUNG Hyun-Jong⁽¹⁾ ; LEE Ki-Yong⁽¹⁾ ; KIM Dong-Il⁽¹⁾ ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾Department of Biological Engineering, Inha University, Incheon 402-751, COREE, REPUBLIQUE DE
R?sum? / Abstract

The production of hGM-CSF was investigated in both a flask and a 5-1 bioreactor, using transgenic Nicotiana tabacum suspension cells. While the maximum cell density and secreted hGM-CSF in the flask were 15.4 g I ^-1 and 6.5 μg l^-1, respectively, those in the bioreactor were 15.6 g l^-1 and 7.6 μg l^-1. No detectable growth inhibition, shorter production of hGM-CSF and reduced cell viability in the batch bioreactor were observed under the specific conditions used compared with the flask culture. To improve the productivity, a perfusion culture was carried out in the bioreactor, with three different perfusion rates (0.5, 1.0 and 2.0 day^-1). In all cases, the hGM-CSF in the medium was significantly increased during the overall culture period (16 days), with maximum values 3.0-, 9.4- and 6.0-fold higher than those obtained in the batch cultures, respectively, even though the intracellular hGM-CSF content was not significantly varied by the perfusion rate. In terms of the total amount of hGM-CSF secreted, 205.5, 1073.2 and 1246.3 μg accumulated in the perfusate within 16 days at the perfusion rates of 0.5, 1.0 and 2.0 day^-1, respectively. It was concluded that the beneficial effect of perfusion on the production of hGM-CSF originated from the reduced proteolytic degradation due to the lower protease activity caused by the perfusion. Additionally, the cell growth and physiology in the perfusion culture were somewhat negatively affected by the increased perfusion rate, although the dry cell density steadily increased, and as a result, 19.4, 22.4 and 22.9 g l^-1 of maximum cells were obtained with perfusion rates of 0.5, 1.0 and 2.0 day^-1, respectively. This work highlighted the importance of proteolytic degradation in plant cell cultures for the production of secretory proteins and the feasibility of perfusion strategies for the continuous production of foreign proteins by the prevention of protein loss due to proteolytic enzymes.

http://cat.inist.fr/?aModele=afficheN&cpsidt=16258829

 

[Sally Furniss]

Re: Vaccine. Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.

Expression of influenza A (H5N1) vaccine in barley grains for oral bird immunization