tetano
Editor, Senior Moderator
Vaccine
. 2020 Dec 31;S0264-410X(20)31631-5.
doi: 10.1016/j.vaccine.2020.12.051. Online ahead of print.
Adjuvant effect of type I interferon induced by many but not all commercial influenza vaccines
Sofi Damjanovska[SUP] 1 [/SUP], Carson Smith[SUP] 2 [/SUP], Ismail Sayin[SUP] 1 [/SUP], Christopher J Burant[SUP] 3 [/SUP], Stefan Gravenstein[SUP] 4 [/SUP], David H Canaday[SUP] 5 [/SUP]
Affiliations
Abstract
Background: Seasonal influenza vaccines approved and offered in the United States have varying reported degrees of effectiveness year over year and between manufacturers. Influenza vaccines produced from live virus may include single stranded RNA (ssRNA) that is a potent activator of the innate Toll-like receptor 7 (TLR-7) ligand. Plasmacytoid dendritic cells (pDC) can be activated by ssRNA to produce type I interferons such as IFN-α, which has been shown to have an adjuvant-like effect.
Objective: Our aim was to determine if IFN-α induction in peripheral blood mononuclear cells (PBMCs) exposed to eight different commercial influenza vaccines is a pDC-dependent process mediated through TLR-7 signaling.
Results: We demonstrate the ability of multiple vaccines to induce IFN-α in a TLR-7-dependent fashion. A number of vaccines however lacked IFN-α induction. The significance of these differences between vaccines is unclear, since all the approved vaccine formulations offer some degree of protection.
Keywords: Human; IFN-α; Immunity; Influenza; Plasmacytoid dendritic cells; Vaccine.
. 2020 Dec 31;S0264-410X(20)31631-5.
doi: 10.1016/j.vaccine.2020.12.051. Online ahead of print.
Adjuvant effect of type I interferon induced by many but not all commercial influenza vaccines
Sofi Damjanovska[SUP] 1 [/SUP], Carson Smith[SUP] 2 [/SUP], Ismail Sayin[SUP] 1 [/SUP], Christopher J Burant[SUP] 3 [/SUP], Stefan Gravenstein[SUP] 4 [/SUP], David H Canaday[SUP] 5 [/SUP]
Affiliations
- PMID: 33390292
- DOI: 10.1016/j.vaccine.2020.12.051
Abstract
Background: Seasonal influenza vaccines approved and offered in the United States have varying reported degrees of effectiveness year over year and between manufacturers. Influenza vaccines produced from live virus may include single stranded RNA (ssRNA) that is a potent activator of the innate Toll-like receptor 7 (TLR-7) ligand. Plasmacytoid dendritic cells (pDC) can be activated by ssRNA to produce type I interferons such as IFN-α, which has been shown to have an adjuvant-like effect.
Objective: Our aim was to determine if IFN-α induction in peripheral blood mononuclear cells (PBMCs) exposed to eight different commercial influenza vaccines is a pDC-dependent process mediated through TLR-7 signaling.
Results: We demonstrate the ability of multiple vaccines to induce IFN-α in a TLR-7-dependent fashion. A number of vaccines however lacked IFN-α induction. The significance of these differences between vaccines is unclear, since all the approved vaccine formulations offer some degree of protection.
Keywords: Human; IFN-α; Immunity; Influenza; Plasmacytoid dendritic cells; Vaccine.