tetano
Editor, Senior Moderator
Vaccine
. 2021 Aug 3;S0264-410X(21)00914-2.
doi: 10.1016/j.vaccine.2021.07.037. Online ahead of print.
A M2 protein-based universal influenza vaccine containing Advax-SM adjuvant provides newborn protection via maternal or neonatal immunization
Isaac G Sakala[SUP] 1 [/SUP], Yoshikazu Honda-Okubo[SUP] 1 [/SUP], Lei Li[SUP] 1 [/SUP], Jeremy Baldwin[SUP] 2 [/SUP], Nikolai Petrovsky[SUP] 3 [/SUP]
Affiliations
Abstract
Background: Despite newborns being at increased risk of serious influenza infection, influenza vaccines are currently not recommended for use in infants under 6 months of age. We therefore sought to evaluate the protective efficacy in mice of an M2-based influenza vaccine (CapM2e) formulated with Advax-SM adjuvant. Vaccine protection was assessed via both passive maternal immunization and direct neonatal immunization.
Methods: For maternal transfer studies, female mice were immunized 1 week before and after mating. Blood was collected from both mother and offspring during weaning and pups were challenged when they reached 3 weeks of age with lethal doses of H1N1 and homologous reassortment influenza strain H3N2 with conserved M2. For direct immunization studies, newborns were immunized at 1 and 3 weeks of age and blood was collected prior to challenge at 4 weeks of age.
Results: Maternal immunization with CapM2e + Advax-SM vaccine induced high maternal M2e antibody levels that were passively transferred to their offspring and provided them with protection against both H1N1 and H3N2 influenza strains when challenged at 3 weeks of age. When used for direct immunization of neonatal mice, CapM2e + Advax-SM vaccine similarly induced high serum M2e antibody levels and protected against H1N1 and H3N2 influenza challenges with protection associated with inhibition of virus replication with a significant reduction in lung virus load in immunized pups.
Conclusion: CapM2e + Advax-SM vaccine could be useful for protecting newborns against diverse influenza A strains, with opportunities to achieve protection by passive maternal immunization or active neonatal immunization. This data supports further development of this promising M2e-based vaccine candidate.
Keywords: Adjuvant; Advax; Influenza; Maternal; Neonatal; Vaccine.
. 2021 Aug 3;S0264-410X(21)00914-2.
doi: 10.1016/j.vaccine.2021.07.037. Online ahead of print.
A M2 protein-based universal influenza vaccine containing Advax-SM adjuvant provides newborn protection via maternal or neonatal immunization
Isaac G Sakala[SUP] 1 [/SUP], Yoshikazu Honda-Okubo[SUP] 1 [/SUP], Lei Li[SUP] 1 [/SUP], Jeremy Baldwin[SUP] 2 [/SUP], Nikolai Petrovsky[SUP] 3 [/SUP]
Affiliations
- PMID: 34362601
- DOI: 10.1016/j.vaccine.2021.07.037
Abstract
Background: Despite newborns being at increased risk of serious influenza infection, influenza vaccines are currently not recommended for use in infants under 6 months of age. We therefore sought to evaluate the protective efficacy in mice of an M2-based influenza vaccine (CapM2e) formulated with Advax-SM adjuvant. Vaccine protection was assessed via both passive maternal immunization and direct neonatal immunization.
Methods: For maternal transfer studies, female mice were immunized 1 week before and after mating. Blood was collected from both mother and offspring during weaning and pups were challenged when they reached 3 weeks of age with lethal doses of H1N1 and homologous reassortment influenza strain H3N2 with conserved M2. For direct immunization studies, newborns were immunized at 1 and 3 weeks of age and blood was collected prior to challenge at 4 weeks of age.
Results: Maternal immunization with CapM2e + Advax-SM vaccine induced high maternal M2e antibody levels that were passively transferred to their offspring and provided them with protection against both H1N1 and H3N2 influenza strains when challenged at 3 weeks of age. When used for direct immunization of neonatal mice, CapM2e + Advax-SM vaccine similarly induced high serum M2e antibody levels and protected against H1N1 and H3N2 influenza challenges with protection associated with inhibition of virus replication with a significant reduction in lung virus load in immunized pups.
Conclusion: CapM2e + Advax-SM vaccine could be useful for protecting newborns against diverse influenza A strains, with opportunities to achieve protection by passive maternal immunization or active neonatal immunization. This data supports further development of this promising M2e-based vaccine candidate.
Keywords: Adjuvant; Advax; Influenza; Maternal; Neonatal; Vaccine.