Mary Wilson
Well-known member
Published: April 04, 2023
DOI: https://doi.org/10.1016/j.celrep.2023.112395
Inga Kavazović, Christoforos Dimitropoulos, Dora Gašparini, Mari Rončević Filipović, Igor Barković, Jan Koster, Niels A. Lemmermann, Marina Babič, Đurđica Cekinović Grbeša, Felix M. Wensveen
Summary
Memory CD8 T cells play an important role in the protection against breakthrough infections with SARS-CoV-2. Whether the route of antigen exposure impacts these cells at a functional level is incompletely characterized. Here we compared the memory CD8 T cell response against a common SARS-CoV-2 epitope after vaccination, infection, or both. CD8 T cells demonstrated comparable functional capacity when restimulated directly ex vivo independent of the antigenic history. However, analysis of T cell receptor usage showed that vaccination resulted in a narrower scope than infection alone or in combination with vaccination. Importantly, in a new in vivo recall model, memory CD8 T cells from infected individuals showed equal proliferation but secreted less TNF compared to those from vaccinated people. This difference was negated when infected individuals had also been vaccinated. Our findings shed more light on the differences in susceptibility to reinfection after different routes of SARS-CoV-2 antigen exposure.
https://www.cell.com/action/showPdf?pii=S2211-1247(23)00406-0
DOI: https://doi.org/10.1016/j.celrep.2023.112395
Inga Kavazović, Christoforos Dimitropoulos, Dora Gašparini, Mari Rončević Filipović, Igor Barković, Jan Koster, Niels A. Lemmermann, Marina Babič, Đurđica Cekinović Grbeša, Felix M. Wensveen
Summary
Memory CD8 T cells play an important role in the protection against breakthrough infections with SARS-CoV-2. Whether the route of antigen exposure impacts these cells at a functional level is incompletely characterized. Here we compared the memory CD8 T cell response against a common SARS-CoV-2 epitope after vaccination, infection, or both. CD8 T cells demonstrated comparable functional capacity when restimulated directly ex vivo independent of the antigenic history. However, analysis of T cell receptor usage showed that vaccination resulted in a narrower scope than infection alone or in combination with vaccination. Importantly, in a new in vivo recall model, memory CD8 T cells from infected individuals showed equal proliferation but secreted less TNF compared to those from vaccinated people. This difference was negated when infected individuals had also been vaccinated. Our findings shed more light on the differences in susceptibility to reinfection after different routes of SARS-CoV-2 antigen exposure.
https://www.cell.com/action/showPdf?pii=S2211-1247(23)00406-0