Mary Wilson
Well-known member
EUROPEAN FOOD SAFETY AUTHORITY
Published: 18 April 2024
DOI https://doi.org/10.2903/j.efsa.2024.8755
EFSA Panel on Animal Health and Animal Welfare (AHAW) | European Union Reference Laboratory for Avian Influenza| Søren Saxmose Nielsen| Julio Alvarez |Dominique Joseph Bicout| Paolo Calistri| Elisabetta Canali| Julian Ashley Drewe|Bruno Garin- Bastuji | Christian Gortázar| Mette S. Herskin | Virginie Michel|Miguel Ángel Miranda Chueca| Barbara Padalino| Helen Clare Roberts | Hans Spoolder |Karl Stahl | Antonio Velarde| Arvo Viltrop | Christoph Winckler| Alessio Bortolami|Claire Guinat| Timm Harder| Arjan Stegeman| Calogero Terregino| Barbara Lanfranchi|Ludovica Preite| Inma Aznar| Alessandro Broglia| Francesca Baldinelli |Jose Luis Gonzales Rojas
Abstract
Selecting appropriate diagnostic methods that take account of the type of vaccine used is important when implementing a vaccination programme against highly pathogenic avian influenza (HPAI). If vaccination is effective, a decreased viral load is expected in the samples used for diagnosis, making molecular methods with high sensitivity the best choice. Although serological methods can be reasonably sensitive, they may produce results that are difficult to interpret. In addition to rou-tine molecular monitoring, it is recommended to conduct viral isolation, genetic sequencing and phenotypic characterisation of any HPAI virus detected in vac-cinated flocks to detect escape mutants early. Following emergency vaccination, various surveillance options based on virological testing of dead birds (‘bucket sampling’) at defined intervals were assessed to be effective for early detection of HPAIV and prove disease freedom in vaccinated populations. For ducks, virological or serological testing of live birds was assessed as an effective strategy. This sur-veillance could be also applied in the peri-vaccination zone on vaccinated estab-lishments, while maintaining passive surveillance in unvaccinated chicken layers and turkeys, and weekly bucket sampling in unvaccinated ducks. To demonstrate disease freedom with > 99% confidence and to detect HPAI virus sufficiently early following preventive vaccination, monthly virological testing of all dead birds up to 15 per flock, coupled with passive surveillance in both vaccinated and unvacci-nated flocks, is recommended. Reducing the sampling intervals increases the sen-sitivity of early detection up to 100%. To enable the safe movement of vaccinated poultry during emergency vaccination, laboratory examinations in the 72 h prior to the movement can be considered as a risk mitigation measure, in addition to clinical inspection; sampling results from existing surveillance activities carried out in these 72 h could be used. In this Opinion, several schemes are recommended to enable the safe movement of vaccinated poultry following preventive vaccination.
https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2024.8755