tetano
Editor, Senior Moderator
J Vis Exp. 2011 Apr 14;(50). pii: 2357. doi: 10.3791/2357.
Using Bioluminescent Imaging to Investigate Synergism Between <em>Streptococcus pneumoniae</em> and Influenza A Virus in Infant Mice.
Short KR, Diavatopoulos DA, Reading PC, Brown LE, Rogers KL, Strugnell RA, Wijburg OL.
Source
Department of Microbiology and Immunology, University of Melbourne.
Abstract
During the 1918 influenza virus pandemic, which killed approximately 50 million people worldwide, the majority of fatalities were not the result of infection with influenza virus alone. Instead, most individuals are thought to have succumbed to a secondary bacterial infection, predominately caused by the bacterium Streptococcus pneumoniae (the pneumococcus). The synergistic relationship between infections caused by influenza virus and the pneumococcus has subsequently been observed during the 1957 Asian influenza virus pandemic, as well as during seasonal outbreaks of the virus (reviewed in (1, 2)). Here, we describe a protocol used to investigate the mechanism(s) that may be involved in increased morbidity as a result of concurrent influenza A virus and S. pneumoniae infection. We have developed an infant murine model to reliably and reproducibly demonstrate the effects of influenza virus infection of mice colonised with S. pneumoniae. Using this protocol, we have provided the first insight into the kinetics of pneumococcal transmission between co-housed, neonatal mice using in vivo imaging (3).
PMID:
21525841
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21525841
Using Bioluminescent Imaging to Investigate Synergism Between <em>Streptococcus pneumoniae</em> and Influenza A Virus in Infant Mice.
Short KR, Diavatopoulos DA, Reading PC, Brown LE, Rogers KL, Strugnell RA, Wijburg OL.
Source
Department of Microbiology and Immunology, University of Melbourne.
Abstract
During the 1918 influenza virus pandemic, which killed approximately 50 million people worldwide, the majority of fatalities were not the result of infection with influenza virus alone. Instead, most individuals are thought to have succumbed to a secondary bacterial infection, predominately caused by the bacterium Streptococcus pneumoniae (the pneumococcus). The synergistic relationship between infections caused by influenza virus and the pneumococcus has subsequently been observed during the 1957 Asian influenza virus pandemic, as well as during seasonal outbreaks of the virus (reviewed in (1, 2)). Here, we describe a protocol used to investigate the mechanism(s) that may be involved in increased morbidity as a result of concurrent influenza A virus and S. pneumoniae infection. We have developed an infant murine model to reliably and reproducibly demonstrate the effects of influenza virus infection of mice colonised with S. pneumoniae. Using this protocol, we have provided the first insight into the kinetics of pneumococcal transmission between co-housed, neonatal mice using in vivo imaging (3).
PMID:
21525841
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21525841