tetano
Editor, Senior Moderator
Indian J Med Microbiol. 2019 Jan-Mar;37(1):105-108. doi: 10.4103/ijmm.IJMM_18_146.
[h=1]Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India.[/h] Gaur B[SUP]1[/SUP], Saha S[SUP]2[/SUP], Iuliano AD[SUP]3[/SUP], Rai SK[SUP]4[/SUP], Krishnan A[SUP]4[/SUP], Jain S[SUP]2[/SUP], Whitaker B[SUP]5[/SUP], Winchell J[SUP]6[/SUP], Lal RB[SUP]2[/SUP], Broor S[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology, All Institute of Medical Sciences, New Delhi, India. 2 Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India. 3 Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 4 Centre for Community Medicine, All Institute of Medical Sciences, New Delhi, India. 5 Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 6 Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
[h=3]Abstract[/h] Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
[h=4]KEYWORDS:[/h] Micro-fluidic card; sensitivity; specificity; viral pathogen diagnosis
PMID: 31424019 DOI: 10.4103/ijmm.IJMM_18_146
[h=1]Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India.[/h] Gaur B[SUP]1[/SUP], Saha S[SUP]2[/SUP], Iuliano AD[SUP]3[/SUP], Rai SK[SUP]4[/SUP], Krishnan A[SUP]4[/SUP], Jain S[SUP]2[/SUP], Whitaker B[SUP]5[/SUP], Winchell J[SUP]6[/SUP], Lal RB[SUP]2[/SUP], Broor S[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology, All Institute of Medical Sciences, New Delhi, India. 2 Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India. 3 Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 4 Centre for Community Medicine, All Institute of Medical Sciences, New Delhi, India. 5 Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 6 Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
[h=3]Abstract[/h] Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
[h=4]KEYWORDS:[/h] Micro-fluidic card; sensitivity; specificity; viral pathogen diagnosis
PMID: 31424019 DOI: 10.4103/ijmm.IJMM_18_146