tetano
Editor, Senior Moderator
J Forensic Sci. 2017 Jan 25. doi: 10.1111/1556-4029.13415. [Epub ahead of print]
[h=1]Use of an Automated Nested Multiplex Respiratory Pathogen PCR Panel Postmortem in the Pediatric Forensic Setting.[/h] Baker T[SUP]1[/SUP], Schandl C[SUP]1[/SUP], Presnell SE[SUP]1[/SUP], Madory J[SUP]1[/SUP], Nolte FS[SUP]1[/SUP], Batalis N[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Respiratory pathogens have been detected in forensic investigations using multiple techniques; however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR), commonly used in living patients, in the forensic setting. This retrospective study assessed the utility of ANM-PCR in detecting respiratory pathogens in the pediatric forensic setting. Respiratory samples from 35 cases were tested for up to 20 respiratory pathogens. 51.4% of these cases yielded a positive ANM-PCR result, 20% of which were considered the cause of or contributory to death. The most commonly detected pathogens were rhinovirus/enterovirus and respiratory syncytial virus, and these were the only pathogens determined to play a significant role in cause of death. The sampled sites and postmortem intervals tested did not affect the likelihood of a positive or negative test. ANM-PCR panels are effective, affordable, and rapid ancillary tools in evaluating cause of death in the forensic pediatric population.
? 2017 American Academy of Forensic Sciences.
[h=4]KEYWORDS:[/h] adenovirus; enterovirus; forensic science; influenza; multiplex polymerase chain reaction; nested polymerase chain reaction; pediatrics; respiratory infection; respiratory syncytial virus; rhinovirus
PMID: 28120330 DOI: 10.1111/1556-4029.13415
[PubMed - as supplied by publisher]
[h=1]Use of an Automated Nested Multiplex Respiratory Pathogen PCR Panel Postmortem in the Pediatric Forensic Setting.[/h] Baker T[SUP]1[/SUP], Schandl C[SUP]1[/SUP], Presnell SE[SUP]1[/SUP], Madory J[SUP]1[/SUP], Nolte FS[SUP]1[/SUP], Batalis N[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Respiratory pathogens have been detected in forensic investigations using multiple techniques; however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR), commonly used in living patients, in the forensic setting. This retrospective study assessed the utility of ANM-PCR in detecting respiratory pathogens in the pediatric forensic setting. Respiratory samples from 35 cases were tested for up to 20 respiratory pathogens. 51.4% of these cases yielded a positive ANM-PCR result, 20% of which were considered the cause of or contributory to death. The most commonly detected pathogens were rhinovirus/enterovirus and respiratory syncytial virus, and these were the only pathogens determined to play a significant role in cause of death. The sampled sites and postmortem intervals tested did not affect the likelihood of a positive or negative test. ANM-PCR panels are effective, affordable, and rapid ancillary tools in evaluating cause of death in the forensic pediatric population.
? 2017 American Academy of Forensic Sciences.
[h=4]KEYWORDS:[/h] adenovirus; enterovirus; forensic science; influenza; multiplex polymerase chain reaction; nested polymerase chain reaction; pediatrics; respiratory infection; respiratory syncytial virus; rhinovirus
PMID: 28120330 DOI: 10.1111/1556-4029.13415
[PubMed - as supplied by publisher]