tetano
Editor, Senior Moderator
J Vet Sci. 2015 Dec 31. [Epub ahead of print]
[h=1]Uracil N-glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination.[/h] Kim EM[SUP]1,[/SUP][SUP]2[/SUP], Jeon HS[SUP]3[/SUP], Kim JJ[SUP]3[/SUP], Shin YK[SUP]4[/SUP], Lee YJ[SUP]5[/SUP], Yeo SG[SUP]1[/SUP], Park CK[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Here, we describe a uracil N-glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that of the RT-LAMP without UNG treatment. To the best of our knowledge, this is the first evidence of using dUTP/UNG strategy in RT-LAMP for AIV detection, and the assay can be applied for the rapid, and reliable diagnosis of AIVs, even in contaminated samples.
[h=4]KEYWORDS:[/h] avian influenza virus; contamination; loop-mediated isothermal amplification; uracil N-glycosylase
PMID: 26726027 [PubMed - as supplied by publisher]
[h=1]Uracil N-glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination.[/h] Kim EM[SUP]1,[/SUP][SUP]2[/SUP], Jeon HS[SUP]3[/SUP], Kim JJ[SUP]3[/SUP], Shin YK[SUP]4[/SUP], Lee YJ[SUP]5[/SUP], Yeo SG[SUP]1[/SUP], Park CK[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Here, we describe a uracil N-glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that of the RT-LAMP without UNG treatment. To the best of our knowledge, this is the first evidence of using dUTP/UNG strategy in RT-LAMP for AIV detection, and the assay can be applied for the rapid, and reliable diagnosis of AIVs, even in contaminated samples.
[h=4]KEYWORDS:[/h] avian influenza virus; contamination; loop-mediated isothermal amplification; uracil N-glycosylase
PMID: 26726027 [PubMed - as supplied by publisher]