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Sensors (Basel). 2019 Oct 17;19(20). pii: E4502. doi: 10.3390/s19204502. [h=1]Ultrasensitive Electrical Detection of Hemagglutinin for Point-of-Care Detection of Influenza Virus Based on a CMP-NANA Probe and Top-Down Processed Silicon Nanowire Field-Effect Transistors.[/h]
Uhm M[SUP]1[/SUP], Lee JM[SUP]2[/SUP], Lee J[SUP]3[/SUP], Lee JH[SUP]4[/SUP], Choi S[SUP]5[/SUP], Park BG[SUP]6[/SUP], Kim DM[SUP]7[/SUP], Choi SJ[SUP]8[/SUP], Mo HS[SUP]9[/SUP], Jeong YJ[SUP]10[/SUP], Kim DH[SUP]11[/SUP].
[h=3]Author information[/h] 1 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. iljss88@naver.com. 2 School of Applied Chemistry, Kookmin University, Seoul 02707, Korea. elzem@kookmin.ac.kr. 3 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. taiji11jieun@kookmin.ac.kr. 4 School of Electrical and Computer Engineering, University of Seoul, Seoul 02504, Korea. kusa159@snu.ac.kr. 5 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. sungjuchoi@kookmin.ac.kr. 6 School of Electrical and Computer Engineering, University of Seoul, Seoul 02504, Korea. bgpark@snu.ac.kr. 7 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. dmkim@kookmin.ac.kr. 8 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. sjchoiee@kookmin.ac.kr. 9 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. tyche@kookmin.ac.kr. 10 School of Applied Chemistry, Kookmin University, Seoul 02707, Korea. jeongyj@kookmin.ac.kr. 11 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. drlife@kookmin.ac.kr.
[h=3]Abstract[/h] Rather than the internal genome nucleic acids, the biomolecules on the surface of the influenza virus itself should be detected for a more exact and rapid point-of-care yes/no decision for influenza virus-induced infectious diseases. This work demonstrates the ultrasensitive electrical detection of the HA1 domain of hemagglutinin (HA), a representative viral surface protein of the influenza virus, using the top-down complementary metal oxide semiconductor (CMOS) processed silicon nanowire (SiNW) field-effect transistor (FET) configuration. Cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NANA) was employed as a probe that specifically binds both to the aldehyde self-aligned monolayer on the SiNWs and to HA1 simultaneously. CMP-NANA was serially combined with two kinds of linkers, namely 3-aminopropyltriethoxysilane and glutaraldehyde. The surface functionalization used was verified using the purification of glutathione S-transferase-tagged HA1, contact angle measurement, enzyme-linked immunosorbent assay test, and isoelectric focusing analysis. The proposed functionalized SiNW FET showed high sensitivities of the threshold voltage shift (ΔV[SUB]T[/SUB]) ~51 mV/pH and the ΔV[SUB]T[/SUB] = 112 mV (63 mV/decade) with an ultralow detectable range of 1 fM of target protein HA1.
[h=4]KEYWORDS:[/h] HA1; hemagglutinin; influenza virus; label-free; point-of-care; silicon nanowire biosensor
PMID: 31627298 DOI: 10.3390/s19204502
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Uhm M[SUP]1[/SUP], Lee JM[SUP]2[/SUP], Lee J[SUP]3[/SUP], Lee JH[SUP]4[/SUP], Choi S[SUP]5[/SUP], Park BG[SUP]6[/SUP], Kim DM[SUP]7[/SUP], Choi SJ[SUP]8[/SUP], Mo HS[SUP]9[/SUP], Jeong YJ[SUP]10[/SUP], Kim DH[SUP]11[/SUP].
[h=3]Author information[/h] 1 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. iljss88@naver.com. 2 School of Applied Chemistry, Kookmin University, Seoul 02707, Korea. elzem@kookmin.ac.kr. 3 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. taiji11jieun@kookmin.ac.kr. 4 School of Electrical and Computer Engineering, University of Seoul, Seoul 02504, Korea. kusa159@snu.ac.kr. 5 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. sungjuchoi@kookmin.ac.kr. 6 School of Electrical and Computer Engineering, University of Seoul, Seoul 02504, Korea. bgpark@snu.ac.kr. 7 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. dmkim@kookmin.ac.kr. 8 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. sjchoiee@kookmin.ac.kr. 9 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. tyche@kookmin.ac.kr. 10 School of Applied Chemistry, Kookmin University, Seoul 02707, Korea. jeongyj@kookmin.ac.kr. 11 School of Electrical Engineering, Kookmin University, Seoul 02707, Korea. drlife@kookmin.ac.kr.
[h=3]Abstract[/h] Rather than the internal genome nucleic acids, the biomolecules on the surface of the influenza virus itself should be detected for a more exact and rapid point-of-care yes/no decision for influenza virus-induced infectious diseases. This work demonstrates the ultrasensitive electrical detection of the HA1 domain of hemagglutinin (HA), a representative viral surface protein of the influenza virus, using the top-down complementary metal oxide semiconductor (CMOS) processed silicon nanowire (SiNW) field-effect transistor (FET) configuration. Cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NANA) was employed as a probe that specifically binds both to the aldehyde self-aligned monolayer on the SiNWs and to HA1 simultaneously. CMP-NANA was serially combined with two kinds of linkers, namely 3-aminopropyltriethoxysilane and glutaraldehyde. The surface functionalization used was verified using the purification of glutathione S-transferase-tagged HA1, contact angle measurement, enzyme-linked immunosorbent assay test, and isoelectric focusing analysis. The proposed functionalized SiNW FET showed high sensitivities of the threshold voltage shift (ΔV[SUB]T[/SUB]) ~51 mV/pH and the ΔV[SUB]T[/SUB] = 112 mV (63 mV/decade) with an ultralow detectable range of 1 fM of target protein HA1.
[h=4]KEYWORDS:[/h] HA1; hemagglutinin; influenza virus; label-free; point-of-care; silicon nanowire biosensor
PMID: 31627298 DOI: 10.3390/s19204502
Free full text