tetano
Editor, Senior Moderator
Anal Chim Acta. 2019 Apr 11;1053:139-147. doi: 10.1016/j.aca.2018.11.056. Epub 2018 Dec 7.
[h=1]Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips.[/h] Xiao M[SUP]1[/SUP], Xie K[SUP]1[/SUP], Dong X[SUP]2[/SUP], Wang L[SUP]1[/SUP], Huang C[SUP]1[/SUP], Xu F[SUP]1[/SUP], Xiao W[SUP]1[/SUP], Jin M[SUP]3[/SUP], Huang B[SUP]4[/SUP], Tang Y[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The development of biosensors that are portable, low-cost, and quantitative has long been sought for rapid, on-site, and timely detection of avian influenza virus (AIV). In this study, an antibody-based Raman lateral flow immunoassay strip was developed to detect AIV H7N9. This LFIA strip used a novel core-shell structure material, AuAg[SUP]4-ATP[/SUP]@AgNPs, as a Raman probe. An antibody specific for AIV and goat anti-mouse IgG antibody were immobilized on a nitrocellulose membrane as the test and control lines, respectively. Accumulation of antibody-virus-antibody-Raman probe complex at the test line could be visualized by the naked eye, and the Raman signal could be quantified using a portable Raman instrument. The testing process for the SERS-based LFIA strips could be completed in 20 min, which avoided the time-cost of current methods for AIV analysis. In our SERS-based biosensor, we estimated the limit of detection (LOD) for H7N9 to be 0.0018 HAU. This value is approximately three orders of magnitude more sensitive than the corresponding HA assays. When testing real sample, the results of the strip test were in accordance with those from real-time PCR testing. In conclusion, the SERS-based LFIA strip proposed in this study shows tremendous potential to detect targets quickly and sensitively using an elegantly simple method.
Copyright ? 2018 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] AuAg(4−ATP)@AgNPs; Avian influenza virus; Lateral flow immunoassay strips; Surface-enhanced Raman scattering
PMID: 30712559 DOI: 10.1016/j.aca.2018.11.056
[h=1]Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips.[/h] Xiao M[SUP]1[/SUP], Xie K[SUP]1[/SUP], Dong X[SUP]2[/SUP], Wang L[SUP]1[/SUP], Huang C[SUP]1[/SUP], Xu F[SUP]1[/SUP], Xiao W[SUP]1[/SUP], Jin M[SUP]3[/SUP], Huang B[SUP]4[/SUP], Tang Y[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The development of biosensors that are portable, low-cost, and quantitative has long been sought for rapid, on-site, and timely detection of avian influenza virus (AIV). In this study, an antibody-based Raman lateral flow immunoassay strip was developed to detect AIV H7N9. This LFIA strip used a novel core-shell structure material, AuAg[SUP]4-ATP[/SUP]@AgNPs, as a Raman probe. An antibody specific for AIV and goat anti-mouse IgG antibody were immobilized on a nitrocellulose membrane as the test and control lines, respectively. Accumulation of antibody-virus-antibody-Raman probe complex at the test line could be visualized by the naked eye, and the Raman signal could be quantified using a portable Raman instrument. The testing process for the SERS-based LFIA strips could be completed in 20 min, which avoided the time-cost of current methods for AIV analysis. In our SERS-based biosensor, we estimated the limit of detection (LOD) for H7N9 to be 0.0018 HAU. This value is approximately three orders of magnitude more sensitive than the corresponding HA assays. When testing real sample, the results of the strip test were in accordance with those from real-time PCR testing. In conclusion, the SERS-based LFIA strip proposed in this study shows tremendous potential to detect targets quickly and sensitively using an elegantly simple method.
Copyright ? 2018 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] AuAg(4−ATP)@AgNPs; Avian influenza virus; Lateral flow immunoassay strips; Surface-enhanced Raman scattering
PMID: 30712559 DOI: 10.1016/j.aca.2018.11.056