tetano
Editor, Senior Moderator
J Virol. 2012 Dec 19. [Epub ahead of print]
Tropism and Infectivity of Influenza Virus, Including Highly Pathogenic Avian H5N1 Virus in Ferret Tracheal Differentiated Primary Epithelial Cell Cultures.
Zeng H, Goldsmith C, Maines TR, Belser JA, Gustin KM, Pekosz A, Zaki S, Katz JM, Tumpey TM.
Source
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Disease.
Abstract
Tropism and adaptation of influenza viruses to new hosts is partly dependent on the distribution of the sialic acid (SA) receptors to which the viral hemagglutinin (HA) binds. Ferrets have been established as a valuable in vivo model of influenza virus pathogenesis and transmission because of similarities to humans in the distribution of HA receptors and in clinical signs of infection. In this study, we developed a ferret tracheal differentiated primary epithelial cell culture model that consisted of a layered epithelium structure with ciliated and non-ciliated cells on its apical surface. We found that human-like (α2,6-linked) receptors predominated on ciliated cells, whereas avian-like (α2,3-linked) receptors, which were less abundant, were presented on non-ciliated cells. When we compared the tropism and infectivity of three human (H1 and H3) and two avian (H1 and H5) influenza viruses, we observed that the human influenza viruses primarily infected ciliated cells and replicated efficiently, whereas, a highly pathogenic avian H5N1 virus (A/Vietnam/1203/2004) replicated efficiently within non-ciliated cells despite a low initial infection rate. Furthermore, compared to other influenza viruses tested, VN/1203 virus replicated more efficiently in cells isolated from the lower trachea, and at a higher temperature (37?C) compared with a lower temperature (33?C). VN/1203 virus infection also induced higher levels of immune mediator genes, cell death and virus was recovered from the basolateral side of the cell monolayer. This ferret tracheal differentiated primary epithelial cell culture system provides a valuable in vitro model for studying cellular tropism, infectivity, and pathogenesis of influenza viruses.
PMID:
23255802
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23255802
Tropism and Infectivity of Influenza Virus, Including Highly Pathogenic Avian H5N1 Virus in Ferret Tracheal Differentiated Primary Epithelial Cell Cultures.
Zeng H, Goldsmith C, Maines TR, Belser JA, Gustin KM, Pekosz A, Zaki S, Katz JM, Tumpey TM.
Source
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Disease.
Abstract
Tropism and adaptation of influenza viruses to new hosts is partly dependent on the distribution of the sialic acid (SA) receptors to which the viral hemagglutinin (HA) binds. Ferrets have been established as a valuable in vivo model of influenza virus pathogenesis and transmission because of similarities to humans in the distribution of HA receptors and in clinical signs of infection. In this study, we developed a ferret tracheal differentiated primary epithelial cell culture model that consisted of a layered epithelium structure with ciliated and non-ciliated cells on its apical surface. We found that human-like (α2,6-linked) receptors predominated on ciliated cells, whereas avian-like (α2,3-linked) receptors, which were less abundant, were presented on non-ciliated cells. When we compared the tropism and infectivity of three human (H1 and H3) and two avian (H1 and H5) influenza viruses, we observed that the human influenza viruses primarily infected ciliated cells and replicated efficiently, whereas, a highly pathogenic avian H5N1 virus (A/Vietnam/1203/2004) replicated efficiently within non-ciliated cells despite a low initial infection rate. Furthermore, compared to other influenza viruses tested, VN/1203 virus replicated more efficiently in cells isolated from the lower trachea, and at a higher temperature (37?C) compared with a lower temperature (33?C). VN/1203 virus infection also induced higher levels of immune mediator genes, cell death and virus was recovered from the basolateral side of the cell monolayer. This ferret tracheal differentiated primary epithelial cell culture system provides a valuable in vitro model for studying cellular tropism, infectivity, and pathogenesis of influenza viruses.
PMID:
23255802
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23255802