tetano
Editor, Senior Moderator
Trop Med Infect Dis
. 2023 Jun 19;8(6):326.
doi: 10.3390/tropicalmed8060326. Simultaneous Detection of Influenza A/B, Respiratory Syncytial Virus, and SARS-CoV-2 in Nasopharyngeal Swabs by One-Tube Multiplex Reverse Transcription Polymerase Chain Reaction
Bader S Alotaibi[SUP] 1 [/SUP], Bilal Ahmad Tantry[SUP] 2 [/SUP], Altaf Bandy[SUP] 3 [/SUP], Reyaz Ahmad[SUP] 2 [/SUP], Syed Quibtiya Khursheed[SUP] 4 [/SUP], Arshid Ahmad[SUP] 5 [/SUP], Mohammed Ageeli Hakami[SUP] 1 [/SUP], Naveed Nazir Shah[SUP] 5 [/SUP]
Affiliations
The treatment and outcome of respiratory virus infections differ. SARS-CoV-2, as well as other respiratory viruses such as influenza virus (A and B) and respiratory syncytial virus (RSV), require simultaneous, cost-effective, and rapid differential detection. We used a gold standard five-target single-step RT-PCR to detect influenza viruses, RSV, and SARS-CoV-2, and this method can be extended to detect influenza virus subtypes. As a result, this five-target single-step RT-PCR method is ideal for differentiating respiratory viruses. The 5' nuclease activity of Taq DNA polymerase is used in the real-time reverse transcription PCR assay. The Taq man fast viral 1-step enzyme is a 4× Master mix and five-target primer probe mix that detects influenza A, influenza B, SARS-CoV-2 ORF1ab, respiratory syncytial viruses A/B and actin. When compared with TaqMan [SUP]TM[/SUP] and Invitrogen superscript [SUP]TM[/SUP] III Platinum and the Meril Kit for SARS-CoV-2, the assay demonstrated 100% sensitivity, specificity, and amplification efficiency of 90.1% for target genes. In conclusion, our one-tube multiplex RT-PCR assay offers a rapid and reliable method for the simultaneous detection of influenza A/B, RSV, and SARS-CoV-2 from nasopharyngeal swabs. This assay has the potential to enhance diagnostic capabilities and improve public health responses during respiratory outbreaks, enabling timely interventions and informed decision making.
Keywords: 5-PLEX assay; RSV; SARS-CoV-2; influenza A; influenza B; real-time reverse transcription.
. 2023 Jun 19;8(6):326.
doi: 10.3390/tropicalmed8060326. Simultaneous Detection of Influenza A/B, Respiratory Syncytial Virus, and SARS-CoV-2 in Nasopharyngeal Swabs by One-Tube Multiplex Reverse Transcription Polymerase Chain Reaction
Bader S Alotaibi[SUP] 1 [/SUP], Bilal Ahmad Tantry[SUP] 2 [/SUP], Altaf Bandy[SUP] 3 [/SUP], Reyaz Ahmad[SUP] 2 [/SUP], Syed Quibtiya Khursheed[SUP] 4 [/SUP], Arshid Ahmad[SUP] 5 [/SUP], Mohammed Ageeli Hakami[SUP] 1 [/SUP], Naveed Nazir Shah[SUP] 5 [/SUP]
Affiliations
- PMID: 37368744
- DOI: 10.3390/tropicalmed8060326
The treatment and outcome of respiratory virus infections differ. SARS-CoV-2, as well as other respiratory viruses such as influenza virus (A and B) and respiratory syncytial virus (RSV), require simultaneous, cost-effective, and rapid differential detection. We used a gold standard five-target single-step RT-PCR to detect influenza viruses, RSV, and SARS-CoV-2, and this method can be extended to detect influenza virus subtypes. As a result, this five-target single-step RT-PCR method is ideal for differentiating respiratory viruses. The 5' nuclease activity of Taq DNA polymerase is used in the real-time reverse transcription PCR assay. The Taq man fast viral 1-step enzyme is a 4× Master mix and five-target primer probe mix that detects influenza A, influenza B, SARS-CoV-2 ORF1ab, respiratory syncytial viruses A/B and actin. When compared with TaqMan [SUP]TM[/SUP] and Invitrogen superscript [SUP]TM[/SUP] III Platinum and the Meril Kit for SARS-CoV-2, the assay demonstrated 100% sensitivity, specificity, and amplification efficiency of 90.1% for target genes. In conclusion, our one-tube multiplex RT-PCR assay offers a rapid and reliable method for the simultaneous detection of influenza A/B, RSV, and SARS-CoV-2 from nasopharyngeal swabs. This assay has the potential to enhance diagnostic capabilities and improve public health responses during respiratory outbreaks, enabling timely interventions and informed decision making.
Keywords: 5-PLEX assay; RSV; SARS-CoV-2; influenza A; influenza B; real-time reverse transcription.