tetano
Editor, Senior Moderator
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Oct;24(5):340-2.
[Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody].
[Article in Chinese]
Hu KX, Zhang LP, Ping RJ, Ma XZ, Yang PF, Sun XH, Zhang L.
Chinese Academy of Inspection and Quarantee, Beijing 100025, China.
Abstract
OBJECTIVE: Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus.
METHODS: Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0.2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions.
RESULTS: The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0.1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus.
CONCLUSION: This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing, which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.
PMID: 21280318 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21280318
[Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody].
[Article in Chinese]
Hu KX, Zhang LP, Ping RJ, Ma XZ, Yang PF, Sun XH, Zhang L.
Chinese Academy of Inspection and Quarantee, Beijing 100025, China.
Abstract
OBJECTIVE: Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus.
METHODS: Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0.2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions.
RESULTS: The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0.1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus.
CONCLUSION: This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing, which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.
PMID: 21280318 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21280318