tetano
Editor, Senior Moderator
Vaccine. 2016 Jun 20. pii: S0264-410X(16)30458-3. doi: 10.1016/j.vaccine.2016.06.034. [Epub ahead of print]
[h=1]Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals.[/h] Haralambieva IH[SUP]1[/SUP], Ovsyannikova IG[SUP]1[/SUP], Kennedy RB[SUP]1[/SUP], Zimmermann MT[SUP]2[/SUP], Grill DE[SUP]2[/SUP], Oberg AL[SUP]2[/SUP], Poland GA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination.
[h=4]METHODS:[/h] We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50-74years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination.
[h=4]RESULTS:[/h] Genesets and genes (p-value range 7.92E[SUP]-08[/SUP] to 0.00018, q-value range 0.00019-0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination.
[h=4]CONCLUSION:[/h] Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.
Copyright ? 2016 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Aging; B-lymphocytes; Enzyme-linked immunospot assay; Gene expression; Immunity, Humoral; Influenza A virus, H1N1 subtype; Influenza vaccines; Influenza, Human; RNA; Sequence analysis; Vaccination
PMID: 27317456 [PubMed - as supplied by publisher]
[h=1]Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals.[/h] Haralambieva IH[SUP]1[/SUP], Ovsyannikova IG[SUP]1[/SUP], Kennedy RB[SUP]1[/SUP], Zimmermann MT[SUP]2[/SUP], Grill DE[SUP]2[/SUP], Oberg AL[SUP]2[/SUP], Poland GA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination.
[h=4]METHODS:[/h] We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50-74years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination.
[h=4]RESULTS:[/h] Genesets and genes (p-value range 7.92E[SUP]-08[/SUP] to 0.00018, q-value range 0.00019-0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination.
[h=4]CONCLUSION:[/h] Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.
Copyright ? 2016 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Aging; B-lymphocytes; Enzyme-linked immunospot assay; Gene expression; Immunity, Humoral; Influenza A virus, H1N1 subtype; Influenza vaccines; Influenza, Human; RNA; Sequence analysis; Vaccination
PMID: 27317456 [PubMed - as supplied by publisher]