tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2015 Jul 14. doi: 10.1111/irv.12334. [Epub ahead of print]
[h=1]The therapeutic effects of sodium cromoglycate against influenza A virus H5N1 in mice.[/h] Han D[SUP]1[/SUP], Wei T[SUP]1[/SUP], Zhang S[SUP]1[/SUP], Wang M[SUP]1,[/SUP][SUP]2[/SUP], Tian H[SUP]1[/SUP], Cheng J[SUP]1[/SUP], Xiao J[SUP]2[/SUP], Hu Y[SUP]1[/SUP], Chen M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The number of drug-resistant influenza virus infections continues to increase, mostly due to the growing use of anti-influenza virus drugs such as oseltamivir and amantadine.
[h=4]OBJECTIVES:[/h] To identify the protective role of sodium cromoglycate in mice during influenza virus infection.
[h=4]METHODS:[/h] H5N1 virus-infected mice were treated with the mast cell stabilizer sodium cromoglycate (SCG) to investigate its therapeutic effect. Virus replication and host responses were determined by plaque assay, quantitative PCR, immunohistochemistry and histology.
[h=4]RESULTS:[/h] SCG-treated mice survived better than did PBS-treated mice after H5N1 virus infection. Mild pathological changes with fewer inflammatory cells infiltration and fewer virus antigens were observed in the nose, trachea, and lungs of SCG-treated mice on days 3 and 5 post-infection. However, no significant changes in viral load in the lungs were detected between SCG and PBS treated mice. Furthermore, significantly decreased expression of interleukin-6, tumor necrosis factor-α, toll like receptor 3 and TIR-domain-containing adapter inducing interferon-β were detected in the lungs of SCG treated mice, and no higher expression of interferon-γ were detected.
[h=4]CONCLUSIONS:[/h] These results suggest that SCG has therapeutic roles in H5N1 virus infected mice by alleviating the inflammatory response rather than inhibition viral replication in the lungs. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Sodium cromoglycate; influenza A virus; therapeutic effects
PMID: 26176755 [PubMed - as supplied by publisher]
[h=1]The therapeutic effects of sodium cromoglycate against influenza A virus H5N1 in mice.[/h] Han D[SUP]1[/SUP], Wei T[SUP]1[/SUP], Zhang S[SUP]1[/SUP], Wang M[SUP]1,[/SUP][SUP]2[/SUP], Tian H[SUP]1[/SUP], Cheng J[SUP]1[/SUP], Xiao J[SUP]2[/SUP], Hu Y[SUP]1[/SUP], Chen M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The number of drug-resistant influenza virus infections continues to increase, mostly due to the growing use of anti-influenza virus drugs such as oseltamivir and amantadine.
[h=4]OBJECTIVES:[/h] To identify the protective role of sodium cromoglycate in mice during influenza virus infection.
[h=4]METHODS:[/h] H5N1 virus-infected mice were treated with the mast cell stabilizer sodium cromoglycate (SCG) to investigate its therapeutic effect. Virus replication and host responses were determined by plaque assay, quantitative PCR, immunohistochemistry and histology.
[h=4]RESULTS:[/h] SCG-treated mice survived better than did PBS-treated mice after H5N1 virus infection. Mild pathological changes with fewer inflammatory cells infiltration and fewer virus antigens were observed in the nose, trachea, and lungs of SCG-treated mice on days 3 and 5 post-infection. However, no significant changes in viral load in the lungs were detected between SCG and PBS treated mice. Furthermore, significantly decreased expression of interleukin-6, tumor necrosis factor-α, toll like receptor 3 and TIR-domain-containing adapter inducing interferon-β were detected in the lungs of SCG treated mice, and no higher expression of interferon-γ were detected.
[h=4]CONCLUSIONS:[/h] These results suggest that SCG has therapeutic roles in H5N1 virus infected mice by alleviating the inflammatory response rather than inhibition viral replication in the lungs. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Sodium cromoglycate; influenza A virus; therapeutic effects
PMID: 26176755 [PubMed - as supplied by publisher]