tetano
Editor, Senior Moderator
J Ethnopharmacol. 2014 Aug 4. pii: S0378-8741(14)00570-4. doi: 10.1016/j.jep.2014.07.051. [Epub ahead of print]
The roots of ilex asprella extract lessens acute respiratory distress syndrome in mice induced by influenza virus.
Dai WP1, Li G1, Li X1, Hu QP1, Liu JX1, Zhang FX2, Su ZR1, Lai XP3.
Author information
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE:
In traditional Chinese medicine, the root of Ilex asprella (Hook. & Arn.) Champ. ex Benth. (IA) has been widely used to treat influenza, lung abscess and other diseases in South China for many years.
AIM OF THE STUDY:
The present study is aimed at investigating the treatment effect of IA on acute respiratory distress syndrome (ARDS) induced by the H1N1 virus in mice.
MATERIALS AND METHODS:
After inoculated with several viral doses of influenza A/FM/1/47 H1N1 virus, mice were oral administration of IA extract (500mg/kg and 125mg/kg per day) for five or ten consecutive days respectively. Mice survival rate and clinical condition were observed for 15 days after inoculation. Lung weight, pathological analysis and arterial blood gas analysis were assessed. Lung viral load was quantified by RT-PCR. Moreover, immunological analysis was measured by leukocyte counts and the levels of inflammatory cytokines, including IL-6, IL-10, TNF-α, IFN-γ, MCP-1 and IL-12p70 in serum of mice.
RESULTS:
We found the extract of Ilex asprella at dosages of 500mg/kg could effectively diminish mortality rate, ameliorate the lung edema and inflammation. Administration of IA extract significantly depressed the expression of IL-6, TNF-α and MCP-1, and significantly increased the expression of IL-10 and IFN-γ in serum. Simultaneously, the extract was also found to reduce the lung viral load and improve the pulmonary ventilation.
CONCLUSION:
The present study shows that the extract of IA has the potential in treating ARDS, due to its abilities on attenuation of systemic and pulmonary inflammatory responses and inhibition of viral replication.
Copyright ? 2014. Published by Elsevier Ireland Ltd.
KEYWORDS:
Acute respiratory distress syndrome; Ilex asprella extract; Immunoregulation; Influenza virus; Viral load
PMID:
25102242
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25102242
The roots of ilex asprella extract lessens acute respiratory distress syndrome in mice induced by influenza virus.
Dai WP1, Li G1, Li X1, Hu QP1, Liu JX1, Zhang FX2, Su ZR1, Lai XP3.
Author information
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE:
In traditional Chinese medicine, the root of Ilex asprella (Hook. & Arn.) Champ. ex Benth. (IA) has been widely used to treat influenza, lung abscess and other diseases in South China for many years.
AIM OF THE STUDY:
The present study is aimed at investigating the treatment effect of IA on acute respiratory distress syndrome (ARDS) induced by the H1N1 virus in mice.
MATERIALS AND METHODS:
After inoculated with several viral doses of influenza A/FM/1/47 H1N1 virus, mice were oral administration of IA extract (500mg/kg and 125mg/kg per day) for five or ten consecutive days respectively. Mice survival rate and clinical condition were observed for 15 days after inoculation. Lung weight, pathological analysis and arterial blood gas analysis were assessed. Lung viral load was quantified by RT-PCR. Moreover, immunological analysis was measured by leukocyte counts and the levels of inflammatory cytokines, including IL-6, IL-10, TNF-α, IFN-γ, MCP-1 and IL-12p70 in serum of mice.
RESULTS:
We found the extract of Ilex asprella at dosages of 500mg/kg could effectively diminish mortality rate, ameliorate the lung edema and inflammation. Administration of IA extract significantly depressed the expression of IL-6, TNF-α and MCP-1, and significantly increased the expression of IL-10 and IFN-γ in serum. Simultaneously, the extract was also found to reduce the lung viral load and improve the pulmonary ventilation.
CONCLUSION:
The present study shows that the extract of IA has the potential in treating ARDS, due to its abilities on attenuation of systemic and pulmonary inflammatory responses and inhibition of viral replication.
Copyright ? 2014. Published by Elsevier Ireland Ltd.
KEYWORDS:
Acute respiratory distress syndrome; Ilex asprella extract; Immunoregulation; Influenza virus; Viral load
PMID:
25102242
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25102242