tetano
Editor, Senior Moderator
Equine Vet J. 2014 Apr 28. doi: 10.1111/evj.12290. [Epub ahead of print]
The potential impact of a single amino acid substitution on the efficacy of equine influenza vaccines.
Yamanaka T1, Cullinane A, Gildea S, Bannai H, Nemoto M, Tsujimura K, Kondo T, Matsumura T.
Author information
Abstract
REASONS FOR PERFORMING STUDY:
The protection induced by an equine influenza (EI) vaccine strain depends on its antigenic relatedness to the challenge virus. Although the World Organisation for Animal Health (OIE) recommend that both Florida sublineage clade 1 (Fc1) and clade 2 (Fc2) viruses should be included in EI vaccines, Japanese EI vaccines have not, thus far, been updated to include a Fc2 virus.
OBJECTIVES:
To evaluate the efficacy of antibodies raised against Japanese EI vaccine strains in the neutralisation of recent Fc2 viruses.
STUDY DESIGN:
Antigenic analysis.
METHODS:
Virus neutralisation tests were performed using antisera from experimentally infected horses and from horses that had received a primary course of the currently available vaccines.
RESULTS:
Antiserum raised against the Japanese EI vaccine strain A/equine/La Plata/1993, exhibited poor cross-neutralising activity against the Fc2 viruses isolated recently in Ireland and the UK which have the substitution alanine to valine at position 144 in antigenic site A of the haemagglutinin gene. In contrast the antiserum exhibited good cross-neutralising activity against the Fc2 viruses without the substitution. This finding was supported in experiments with antisera collected from vaccinated horses.
CONCLUSIONS:
This suggests that the efficacy of the Japanese EI vaccine for some of the recent Fc2 viruses is suboptimal and that vaccines should be updated in accordance with the OIE recommendations.
This article is protected by copyright. All rights reserved.
KEYWORDS:
cross-neutralisation, equine antisera, equine influenza, horse, vaccine update
PMID:
24773030
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24773030
The potential impact of a single amino acid substitution on the efficacy of equine influenza vaccines.
Yamanaka T1, Cullinane A, Gildea S, Bannai H, Nemoto M, Tsujimura K, Kondo T, Matsumura T.
Author information
Abstract
REASONS FOR PERFORMING STUDY:
The protection induced by an equine influenza (EI) vaccine strain depends on its antigenic relatedness to the challenge virus. Although the World Organisation for Animal Health (OIE) recommend that both Florida sublineage clade 1 (Fc1) and clade 2 (Fc2) viruses should be included in EI vaccines, Japanese EI vaccines have not, thus far, been updated to include a Fc2 virus.
OBJECTIVES:
To evaluate the efficacy of antibodies raised against Japanese EI vaccine strains in the neutralisation of recent Fc2 viruses.
STUDY DESIGN:
Antigenic analysis.
METHODS:
Virus neutralisation tests were performed using antisera from experimentally infected horses and from horses that had received a primary course of the currently available vaccines.
RESULTS:
Antiserum raised against the Japanese EI vaccine strain A/equine/La Plata/1993, exhibited poor cross-neutralising activity against the Fc2 viruses isolated recently in Ireland and the UK which have the substitution alanine to valine at position 144 in antigenic site A of the haemagglutinin gene. In contrast the antiserum exhibited good cross-neutralising activity against the Fc2 viruses without the substitution. This finding was supported in experiments with antisera collected from vaccinated horses.
CONCLUSIONS:
This suggests that the efficacy of the Japanese EI vaccine for some of the recent Fc2 viruses is suboptimal and that vaccines should be updated in accordance with the OIE recommendations.
This article is protected by copyright. All rights reserved.
KEYWORDS:
cross-neutralisation, equine antisera, equine influenza, horse, vaccine update
PMID:
24773030
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24773030