tetano
Editor, Senior Moderator
Virology. 2019 Jul 8;535:218-226. doi: 10.1016/j.virol.2019.07.001. [Epub ahead of print]
[h=1]The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.[/h] Hao X[SUP]1[/SUP], Wang X[SUP]1[/SUP], Hu J[SUP]1[/SUP], Gu M[SUP]1[/SUP], Wang J[SUP]1[/SUP], Deng Y[SUP]1[/SUP], Jiang D[SUP]1[/SUP], He D[SUP]1[/SUP], Xu H[SUP]1[/SUP], Yang Y[SUP]1[/SUP], Hu Z[SUP]1[/SUP], Chen S[SUP]1[/SUP], Hu S[SUP]1[/SUP], Liu X[SUP]1[/SUP], Shang S[SUP]1[/SUP], Peng D[SUP]1[/SUP], Jiao X[SUP]2[/SUP], Liu X[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Genotype S H9N2 viruses frequently donate their internal genes to facilitate the generation of novel influenza viruses, e.g., H5N6, H7N9, and H10N8, which have caused human infection. Genotype S was originated from the replacement of F/98-like M and PB2 genes of the genotype H with those from G1-like lineage. However, whether this gene substitution will influence the viral fitness of emerging influenza viruses remains unclear. We found that H5Nx and H7N9 viruses with G1-like PB2 or M gene exhibited higher virulence and replication than those with F/98-like PB2 or M in chickens. We also determined the functional significance of G1-like PB2 in conferring increased polymerase activity and improved nucleus transportation efficiency, and facilitated RNP nuclear export by G1-like M. Our results suggest that G1-like PB2 and M genes optimize viral fitness, and thus play a crucial role in the genesis of emerging influenza viruses that cause rising prevalence in chickens.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chickens; G1-like PB2 and M; H5Nx; H7N9; Viral fitness
PMID: 31325836 DOI: 10.1016/j.virol.2019.07.001
[h=1]The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.[/h] Hao X[SUP]1[/SUP], Wang X[SUP]1[/SUP], Hu J[SUP]1[/SUP], Gu M[SUP]1[/SUP], Wang J[SUP]1[/SUP], Deng Y[SUP]1[/SUP], Jiang D[SUP]1[/SUP], He D[SUP]1[/SUP], Xu H[SUP]1[/SUP], Yang Y[SUP]1[/SUP], Hu Z[SUP]1[/SUP], Chen S[SUP]1[/SUP], Hu S[SUP]1[/SUP], Liu X[SUP]1[/SUP], Shang S[SUP]1[/SUP], Peng D[SUP]1[/SUP], Jiao X[SUP]2[/SUP], Liu X[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Genotype S H9N2 viruses frequently donate their internal genes to facilitate the generation of novel influenza viruses, e.g., H5N6, H7N9, and H10N8, which have caused human infection. Genotype S was originated from the replacement of F/98-like M and PB2 genes of the genotype H with those from G1-like lineage. However, whether this gene substitution will influence the viral fitness of emerging influenza viruses remains unclear. We found that H5Nx and H7N9 viruses with G1-like PB2 or M gene exhibited higher virulence and replication than those with F/98-like PB2 or M in chickens. We also determined the functional significance of G1-like PB2 in conferring increased polymerase activity and improved nucleus transportation efficiency, and facilitated RNP nuclear export by G1-like M. Our results suggest that G1-like PB2 and M genes optimize viral fitness, and thus play a crucial role in the genesis of emerging influenza viruses that cause rising prevalence in chickens.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chickens; G1-like PB2 and M; H5Nx; H7N9; Viral fitness
PMID: 31325836 DOI: 10.1016/j.virol.2019.07.001