tetano
Editor, Senior Moderator
J Mol Biol. 2017 Apr 19. pii: S0022-2836(17)30182-1. doi: 10.1016/j.jmb.2017.04.007. [Epub ahead of print]
[h=1]The NS1 protein from influenza virus stimulates translation initiation by enhancing ribosome recruitment to mRNAs.[/h] Panthu B[SUP]1[/SUP], Terrier O[SUP]2[/SUP], Carron C[SUP]2[/SUP], Traversier A[SUP]2[/SUP], Corbin A[SUP]1[/SUP], Balvay L[SUP]1[/SUP], Lina B[SUP]2[/SUP], Rosa-Calatrava M[SUP]2[/SUP], Ohlmann T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The non-structural protein NS1 of influenza A viruses exerts pleiotropic functions during infection. Amongst these functions, NS1 was shown to be involved both in the control of viral and cellular translation; however the mechanism by which this occurs remains to be determined. Thus, we have revisited the role of NS1 in translation by using a combination of influenza infection, mRNA reporter transfection, in vitro functional and biochemical assays. Our data show that the NS1 protein is able to enhance translation of virtually all tested mRNAs with the exception of constructs bearing the Dicistroviruses IRESes (DCV and CrPV), suggesting a role at the level of translation initiation. The domain of NS1 required for translation stimulation was mapped to the RNA binding amino-terminal motif of the protein with residues R38 and K41 being critical for activity. Although we show that NS1 can bind directly to mRNAs, it does not correlate with its ability to stimulate translation. This activity rather relies on the property of NS1 to associate with ribosomes and to recruit them to target mRNAs.
Copyright ? 2017. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Influenza; NS1; RNA; Ribosome; Translation
PMID: 28433538 DOI: 10.1016/j.jmb.2017.04.007
[h=1]The NS1 protein from influenza virus stimulates translation initiation by enhancing ribosome recruitment to mRNAs.[/h] Panthu B[SUP]1[/SUP], Terrier O[SUP]2[/SUP], Carron C[SUP]2[/SUP], Traversier A[SUP]2[/SUP], Corbin A[SUP]1[/SUP], Balvay L[SUP]1[/SUP], Lina B[SUP]2[/SUP], Rosa-Calatrava M[SUP]2[/SUP], Ohlmann T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The non-structural protein NS1 of influenza A viruses exerts pleiotropic functions during infection. Amongst these functions, NS1 was shown to be involved both in the control of viral and cellular translation; however the mechanism by which this occurs remains to be determined. Thus, we have revisited the role of NS1 in translation by using a combination of influenza infection, mRNA reporter transfection, in vitro functional and biochemical assays. Our data show that the NS1 protein is able to enhance translation of virtually all tested mRNAs with the exception of constructs bearing the Dicistroviruses IRESes (DCV and CrPV), suggesting a role at the level of translation initiation. The domain of NS1 required for translation stimulation was mapped to the RNA binding amino-terminal motif of the protein with residues R38 and K41 being critical for activity. Although we show that NS1 can bind directly to mRNAs, it does not correlate with its ability to stimulate translation. This activity rather relies on the property of NS1 to associate with ribosomes and to recruit them to target mRNAs.
Copyright ? 2017. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Influenza; NS1; RNA; Ribosome; Translation
PMID: 28433538 DOI: 10.1016/j.jmb.2017.04.007