tetano
Editor, Senior Moderator
Curr Opin Chem Biol. 2014 Feb 5;18C:87-94. doi: 10.1016/j.cbpa.2014.01.007. [Epub ahead of print]
The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the meta-glycome.
Palma AS1, Feizi T2, Childs RA3, Chai W3, Liu Y4.
Author information
Abstract
The neoglycolipid (NGL) technology is the basis of a state-of-the-art oligosaccharide microarray system. The NGL-based microarray system in the Glycosciences Laboratory Imperial College London (http://www3.imperial.ac.uk/glycosciences) is one of the two leading platforms for glycan microarrays, being offered for screening analyses to the broad biomedical community. Highlighted in this review are the sensitivity of the analysis system and, coupled with mass spectrometry, the provision for generating 'designer' microarrays from glycomes to identify novel ligands of biological relevance. Among recent applications are assignments of ligands for apicomplexan parasites, pandemic 2009 influenza virus, polyoma and reoviruses, an innate immune receptor against fungal pathogens, Dectin-1, and a novel protein of the endoplasmic reticulum, malectin; also the characterization of an elusive cancer-associated antigen. Some other contemporary advances in glycolipid-containing arrays and microarrays are also discussed.
Copyright ? 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
PMID:
24508828
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24508828
The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the meta-glycome.
Palma AS1, Feizi T2, Childs RA3, Chai W3, Liu Y4.
Author information
Abstract
The neoglycolipid (NGL) technology is the basis of a state-of-the-art oligosaccharide microarray system. The NGL-based microarray system in the Glycosciences Laboratory Imperial College London (http://www3.imperial.ac.uk/glycosciences) is one of the two leading platforms for glycan microarrays, being offered for screening analyses to the broad biomedical community. Highlighted in this review are the sensitivity of the analysis system and, coupled with mass spectrometry, the provision for generating 'designer' microarrays from glycomes to identify novel ligands of biological relevance. Among recent applications are assignments of ligands for apicomplexan parasites, pandemic 2009 influenza virus, polyoma and reoviruses, an innate immune receptor against fungal pathogens, Dectin-1, and a novel protein of the endoplasmic reticulum, malectin; also the characterization of an elusive cancer-associated antigen. Some other contemporary advances in glycolipid-containing arrays and microarrays are also discussed.
Copyright ? 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
PMID:
24508828
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24508828