tetano
Editor, Senior Moderator
BMC Res Notes. 2016 May 20;9(1):279. doi: 10.1186/s13104-016-2083-6.
[h=1]The influenza A virus NS genome segment displays lineage-specific patterns in predicted RNA secondary structure.[/h] Vasin AV[SUP]1,[/SUP][SUP]2[/SUP], Petrova AV[SUP]3,[/SUP][SUP]4[/SUP], Egorov VV[SUP]3[/SUP], Plotnikova MA[SUP]3[/SUP], Klotchenko SA[SUP]3[/SUP], Karpenko MN[SUP]4[/SUP], Kiselev OI[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza A virus (IAV) is a segmented negative-sense RNA virus that causes seasonal epidemics and periodic pandemics in humans. Two regions (nucleotide positions 82-148 and 497-564) in the positive-sense RNA of the NS segment fold into a multi-branch loop or hairpin structures.
[h=4]RESULTS:[/h] We studied 25,384 NS segment positive-sense RNA unique sequences of human and non-human IAVs in order to predict secondary RNA structures of the 82-148 and 497-564 regions using RNAfold software, and determined their host- and lineage-specific distributions. Hairpins prevailed in avian and avian-origin human IAVs, including H1N1pdm1918 and H5N1. In human and swine IAV hairpins distribution varied between evolutionary lineages.
[h=4]CONCLUSIONS:[/h] These results suggest a possible functional role for these RNA secondary structures and the need for experimental evaluation of these structures in the influenza life cycle.
[h=4]KEYWORDS:[/h] Evolution; Influenza A virus; NS gene; Pathogenicity; RNA hairpin; RNA secondary structure
PMID: 27206548 [PubMed - in process] PMCID: PMC4875733 Free PMC Article
[h=1]The influenza A virus NS genome segment displays lineage-specific patterns in predicted RNA secondary structure.[/h] Vasin AV[SUP]1,[/SUP][SUP]2[/SUP], Petrova AV[SUP]3,[/SUP][SUP]4[/SUP], Egorov VV[SUP]3[/SUP], Plotnikova MA[SUP]3[/SUP], Klotchenko SA[SUP]3[/SUP], Karpenko MN[SUP]4[/SUP], Kiselev OI[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza A virus (IAV) is a segmented negative-sense RNA virus that causes seasonal epidemics and periodic pandemics in humans. Two regions (nucleotide positions 82-148 and 497-564) in the positive-sense RNA of the NS segment fold into a multi-branch loop or hairpin structures.
[h=4]RESULTS:[/h] We studied 25,384 NS segment positive-sense RNA unique sequences of human and non-human IAVs in order to predict secondary RNA structures of the 82-148 and 497-564 regions using RNAfold software, and determined their host- and lineage-specific distributions. Hairpins prevailed in avian and avian-origin human IAVs, including H1N1pdm1918 and H5N1. In human and swine IAV hairpins distribution varied between evolutionary lineages.
[h=4]CONCLUSIONS:[/h] These results suggest a possible functional role for these RNA secondary structures and the need for experimental evaluation of these structures in the influenza life cycle.
[h=4]KEYWORDS:[/h] Evolution; Influenza A virus; NS gene; Pathogenicity; RNA hairpin; RNA secondary structure
PMID: 27206548 [PubMed - in process] PMCID: PMC4875733 Free PMC Article