tetano
Editor, Senior Moderator
RNA Biol. 2012 Oct 12;9(11). [Epub ahead of print]
The influenza A segment 7 mRNA 3' splice site pseudoknot/hairpin family.
Moss WN, Dela-Moss LI, Priore SF, Turner DH.
Source
Department of Chemistry and Center for RNA Biology; University of Rochester; Rochester, NY USA; Department of Molecular Biophysics and Biochemistry; Howard Hughes Medical Institute; Yale University School of Medicine; New Haven, CT USA.
Abstract
The 3' splice site of the influenza A segment 7 transcript is utilized to produce mRNA for the critical M2 ion-channel protein. In solution a 63 nt fragment that includes this region can adopt two conformations: a pseudoknot and a hairpin. In each conformation, the splice site, a binding site for the SF2/ASF exonic splicing enhancer and a polypyrimidine tract, each exists in a different structural context. The most dramatic difference occurs for the splice site. In the hairpin the splice site is between two residues that are involved in a 2 by 2 nucleotide internal loop. In the pseudoknot, however, these bases are canonically paired within one of the pseudoknotted helices. The conformational switching observed in this region has implications for the regulation of splicing of the segment 7 mRNA. A measure of stability of the structures also shows interesting trends with respect to host specificity: avian strains tend to be the most stable, followed by swine and then human.
PMID:
23064116
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23064116
The influenza A segment 7 mRNA 3' splice site pseudoknot/hairpin family.
Moss WN, Dela-Moss LI, Priore SF, Turner DH.
Source
Department of Chemistry and Center for RNA Biology; University of Rochester; Rochester, NY USA; Department of Molecular Biophysics and Biochemistry; Howard Hughes Medical Institute; Yale University School of Medicine; New Haven, CT USA.
Abstract
The 3' splice site of the influenza A segment 7 transcript is utilized to produce mRNA for the critical M2 ion-channel protein. In solution a 63 nt fragment that includes this region can adopt two conformations: a pseudoknot and a hairpin. In each conformation, the splice site, a binding site for the SF2/ASF exonic splicing enhancer and a polypyrimidine tract, each exists in a different structural context. The most dramatic difference occurs for the splice site. In the hairpin the splice site is between two residues that are involved in a 2 by 2 nucleotide internal loop. In the pseudoknot, however, these bases are canonically paired within one of the pseudoknotted helices. The conformational switching observed in this region has implications for the regulation of splicing of the segment 7 mRNA. A measure of stability of the structures also shows interesting trends with respect to host specificity: avian strains tend to be the most stable, followed by swine and then human.
PMID:
23064116
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23064116