tetano
Editor, Senior Moderator
J Virol. 2013 Aug 7. [Epub ahead of print]
The genome packaging signal of the influenza A virus genome comprises a genome incorporation signal and a genome bundling signal.
Goto H, Muramoto Y, Noda T, Kawaoka Y.
Source
Division of Virology, Department of Microbiology and immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Abstract
The influenza A virus genome comprises eight single-stranded negative-sense RNA segments (vRNAs). All eight vRNAs are selectively packaged into each progeny virion via so-called segment-specific genome packaging signal sequences that are located in the noncoding and terminal coding regions of both the 3' and 5' ends of the vRNAs. However, it remains unclear how these signals ensure that eight different vRNAs are packaged. Here, by using reverse genetics system, we demonstrated that, in the absence of the other 7 vRNAs, a recombinant NP vRNA bearing only a reporter gene flanked by the noncoding NP regions was incorporated into virus-like particles as efficiently as a recombinant NP vRNA bearing the reporter gene flanked by the complete NP packaging signals (i.e., the noncoding sequences and the terminal coding regions). Viruses that comprised a recombinant NP vRNA whose packaging signal was disrupted, and the remaining 7 authentic vRNAs, did not undergo multiple cycles of replication; however, a recombinant NP vRNA with only the noncoding regions was readily incorporated into VLPs, suggesting that the packaging signal as currently defined is not necessarily essential for the packaging of the vRNA in which it resides; rather, it is required for the packaging of the full set of vRNAs. We propose that the 3' and 5' noncoding regions of each vRNA bear a virion incorporation signal for that vRNA and that the terminal coding regions serve as a bundling signal that ensures the incorporation of the complete set of 8 vRNAs into the virion.
PMID:
23926345
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23926345
The genome packaging signal of the influenza A virus genome comprises a genome incorporation signal and a genome bundling signal.
Goto H, Muramoto Y, Noda T, Kawaoka Y.
Source
Division of Virology, Department of Microbiology and immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Abstract
The influenza A virus genome comprises eight single-stranded negative-sense RNA segments (vRNAs). All eight vRNAs are selectively packaged into each progeny virion via so-called segment-specific genome packaging signal sequences that are located in the noncoding and terminal coding regions of both the 3' and 5' ends of the vRNAs. However, it remains unclear how these signals ensure that eight different vRNAs are packaged. Here, by using reverse genetics system, we demonstrated that, in the absence of the other 7 vRNAs, a recombinant NP vRNA bearing only a reporter gene flanked by the noncoding NP regions was incorporated into virus-like particles as efficiently as a recombinant NP vRNA bearing the reporter gene flanked by the complete NP packaging signals (i.e., the noncoding sequences and the terminal coding regions). Viruses that comprised a recombinant NP vRNA whose packaging signal was disrupted, and the remaining 7 authentic vRNAs, did not undergo multiple cycles of replication; however, a recombinant NP vRNA with only the noncoding regions was readily incorporated into VLPs, suggesting that the packaging signal as currently defined is not necessarily essential for the packaging of the vRNA in which it resides; rather, it is required for the packaging of the full set of vRNAs. We propose that the 3' and 5' noncoding regions of each vRNA bear a virion incorporation signal for that vRNA and that the terminal coding regions serve as a bundling signal that ensures the incorporation of the complete set of 8 vRNAs into the virion.
PMID:
23926345
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23926345