tetano
Editor, Senior Moderator
Sci Rep. 2015 Feb 5;5:8262. doi: 10.1038/srep08262.
[h=1]The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses.[/h] Gao H[SUP]1[/SUP], Sun Y[SUP]1[/SUP], Hu J[SUP]2[/SUP], Qi L[SUP]1[/SUP], Wang J[SUP]1[/SUP], Xiong X[SUP]1[/SUP], Wang Y[SUP]1[/SUP], He Q[SUP]1[/SUP], Lin Y[SUP]1[/SUP], Kong W[SUP]1[/SUP], Seng LG[SUP]3[/SUP], Sun H[SUP]1[/SUP], Pu J[SUP]1[/SUP], Chang KC[SUP]3[/SUP], Liu X[SUP]2[/SUP], Liu J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] PA-X is a novel protein encoded by PA mRNA and is found to decrease the pathogenicity of pandemic 1918 H1N1 virus in mice. However, the importance of PA-X proteins in current epidemiologically important influenza A virus strains is not known. In this study, we report on the pathogenicity and pathological effects of PA-X deficient 2009 pandemic H1N1 (pH1N1) and highly pathogenic avian influenza H5N1 viruses. We found that loss of PA-X expression in pH1N1 and H5N1 viruses increased viral replication and apoptosis in A549 cells and increased virulence and host inflammatory response in mice. In addition, PA-X deficient pH1N1 and H5N1 viruses up-regulated PA mRNA and protein synthesis and increased viral polymerase activity. Loss of PA-X was also accompanied by accelerated nuclear accumulation of PA protein and reduced suppression of PA on non-viral protein expression. Our study highlights the effects of PA-X on the moderation of viral pathogenesis and pathogenicity.
PMID: 25652161 [PubMed - in process]
[h=1]The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses.[/h] Gao H[SUP]1[/SUP], Sun Y[SUP]1[/SUP], Hu J[SUP]2[/SUP], Qi L[SUP]1[/SUP], Wang J[SUP]1[/SUP], Xiong X[SUP]1[/SUP], Wang Y[SUP]1[/SUP], He Q[SUP]1[/SUP], Lin Y[SUP]1[/SUP], Kong W[SUP]1[/SUP], Seng LG[SUP]3[/SUP], Sun H[SUP]1[/SUP], Pu J[SUP]1[/SUP], Chang KC[SUP]3[/SUP], Liu X[SUP]2[/SUP], Liu J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] PA-X is a novel protein encoded by PA mRNA and is found to decrease the pathogenicity of pandemic 1918 H1N1 virus in mice. However, the importance of PA-X proteins in current epidemiologically important influenza A virus strains is not known. In this study, we report on the pathogenicity and pathological effects of PA-X deficient 2009 pandemic H1N1 (pH1N1) and highly pathogenic avian influenza H5N1 viruses. We found that loss of PA-X expression in pH1N1 and H5N1 viruses increased viral replication and apoptosis in A549 cells and increased virulence and host inflammatory response in mice. In addition, PA-X deficient pH1N1 and H5N1 viruses up-regulated PA mRNA and protein synthesis and increased viral polymerase activity. Loss of PA-X was also accompanied by accelerated nuclear accumulation of PA protein and reduced suppression of PA on non-viral protein expression. Our study highlights the effects of PA-X on the moderation of viral pathogenesis and pathogenicity.
PMID: 25652161 [PubMed - in process]