tetano
Editor, Senior Moderator
BMC Complement Altern Med. 2017 Feb 24;17(1):130. doi: 10.1186/s12906-017-1585-7.
[h=1]The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function.[/h] Ding Y[SUP]1,[/SUP][SUP]2[/SUP], Zeng L[SUP]1,[/SUP][SUP]2[/SUP], Li R[SUP]1[/SUP], Chen Q[SUP]3[/SUP], Zhou B[SUP]1,[/SUP][SUP]4[/SUP], Chen Q[SUP]1[/SUP], Cheng PL[SUP]5[/SUP], Yutao W[SUP]1[/SUP], Zheng J[SUP]1[/SUP], Yang Z[SUP]6,[/SUP][SUP]7[/SUP], Zhang F[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Lianhuaqingwen Capsule (LH-C) is a traditional Chinese medicine (TCM) formula used to treat respiratory tract infectious diseases in Chinese. The aim of this study was to determine the antiviral activity of LH-C and its immunomodulatory effects on viral infection.
[h=4]METHOD:[/h] The in vitro cytotoxicity and antiviral activity of LH-C was determined by MTT and Plaque reduction assays. Time course study under single-cycle virus growth conditions were used to determine which stage of viral replication was blocked. The effect of LH-C on the nuclear export of the viral nucleoprotein was examined using an indirect immunofluorescence assay. The regulation to different signaling transduction events and cytokine/chemokine expression of LH-C was evaluated using Western blotting and real-time RT-PCR. After virus inoculation, BALB/c mice were administered with LH-C of different concentrations for 5 days. Body-weight, viral titers and lung pathology of the mice were measured, the level of inflammatory cytokines were also examined using real-time RT-PCR.
[h=4]RESULTS:[/h] LH-C inhibited the proliferation of influenza viruses of various strain in vitro, with the 50% inhibitory concentration (IC[SUB]50[/SUB]) ranging from 0.35 to 2 mg/mL. LH-C blocked the early stages (0-2 h) of virus infection, it also suppressed virus-induced NF-kB activation and alleviated virus-induced gene expression of IL-6, IL-8, TNF-a, IP-10, and MCP-1 in a dose-dependent manner. LH-C treatment efficiently impaired the nuclear export of the viral RNP. A decrease of the viral titers in the lungs of mice were observed in groups administered with LH-C. The level of inflammatory cytokines were also decreased in the early stages of infection.
[h=4]CONCLUSIONS:[/h] LH-C, as a TCM prescription, exerts broad-spectrum effects on a series of influenza viruses, including the newly emerged H7N9, and particularly regulates the immune response of virus infection. Thus, LH-C might be a promising option for treating influenza virus infection.
[h=4]KEYWORDS:[/h] Antiviral; Immuno-regulation; Influenza virus; Lianhuaqingwen capsule
PMID: 28235408 DOI: 10.1186/s12906-017-1585-7
[PubMed - in process]
[h=1]The Chinese prescription lianhuaqingwen capsule exerts anti-influenza activity through the inhibition of viral propagation and impacts immune function.[/h] Ding Y[SUP]1,[/SUP][SUP]2[/SUP], Zeng L[SUP]1,[/SUP][SUP]2[/SUP], Li R[SUP]1[/SUP], Chen Q[SUP]3[/SUP], Zhou B[SUP]1,[/SUP][SUP]4[/SUP], Chen Q[SUP]1[/SUP], Cheng PL[SUP]5[/SUP], Yutao W[SUP]1[/SUP], Zheng J[SUP]1[/SUP], Yang Z[SUP]6,[/SUP][SUP]7[/SUP], Zhang F[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Lianhuaqingwen Capsule (LH-C) is a traditional Chinese medicine (TCM) formula used to treat respiratory tract infectious diseases in Chinese. The aim of this study was to determine the antiviral activity of LH-C and its immunomodulatory effects on viral infection.
[h=4]METHOD:[/h] The in vitro cytotoxicity and antiviral activity of LH-C was determined by MTT and Plaque reduction assays. Time course study under single-cycle virus growth conditions were used to determine which stage of viral replication was blocked. The effect of LH-C on the nuclear export of the viral nucleoprotein was examined using an indirect immunofluorescence assay. The regulation to different signaling transduction events and cytokine/chemokine expression of LH-C was evaluated using Western blotting and real-time RT-PCR. After virus inoculation, BALB/c mice were administered with LH-C of different concentrations for 5 days. Body-weight, viral titers and lung pathology of the mice were measured, the level of inflammatory cytokines were also examined using real-time RT-PCR.
[h=4]RESULTS:[/h] LH-C inhibited the proliferation of influenza viruses of various strain in vitro, with the 50% inhibitory concentration (IC[SUB]50[/SUB]) ranging from 0.35 to 2 mg/mL. LH-C blocked the early stages (0-2 h) of virus infection, it also suppressed virus-induced NF-kB activation and alleviated virus-induced gene expression of IL-6, IL-8, TNF-a, IP-10, and MCP-1 in a dose-dependent manner. LH-C treatment efficiently impaired the nuclear export of the viral RNP. A decrease of the viral titers in the lungs of mice were observed in groups administered with LH-C. The level of inflammatory cytokines were also decreased in the early stages of infection.
[h=4]CONCLUSIONS:[/h] LH-C, as a TCM prescription, exerts broad-spectrum effects on a series of influenza viruses, including the newly emerged H7N9, and particularly regulates the immune response of virus infection. Thus, LH-C might be a promising option for treating influenza virus infection.
[h=4]KEYWORDS:[/h] Antiviral; Immuno-regulation; Influenza virus; Lianhuaqingwen capsule
PMID: 28235408 DOI: 10.1186/s12906-017-1585-7
[PubMed - in process]