tetano
Editor, Senior Moderator
J Gen Virol. 2015 May 1. pii: vir.0.000171. doi: 10.1099/vir.0.000171. [Epub ahead of print]
[h=1]The C-terminus of NS1 protein of influenza A/WSN/1933(H1N1) virus modulates antiviral responses in infected human macrophages and mice.[/h] Anastasina M[SUP]1[/SUP], Schepens B[SUP]2[/SUP], S?derholm S[SUP]3[/SUP], Nyman TA[SUP]4[/SUP], Matikainen S[SUP]3[/SUP], Saksela K[SUP]5[/SUP], Saelens X[SUP]2[/SUP], Kainov DE[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Non-structural protein NS1 of influenza A viruses interacts with cellular factors through its N-terminal RNA-binding, middle effector and C-terminal non-structured domains. NS1 attenuates antiviral responses in infected cells and thereby secures efficient virus replication. Some influenza strains express C-terminally truncated NS1 proteins due to nonsense mutations in NS1 gene. To understand the role of NS1 C-terminal region in regulation of antiviral responses, we engineered influenza viruses expressing C-terminally truncated NS1 proteins using A/WSN/33(H1N1) reverse-genetics and tested them in human macrophages and in mice. We showed that WSN virus expressing NS1 with a 28 amino acids deletion from its C-terminus is a more powerful inducer of antiviral responses than the virus expressing full-length NS1 or one with a 10 amino acid truncation of NS1. Thus, our finding suggests that the C-terminal region of NS1 is essential for regulation of antiviral responses. Moreover, viruses expressing truncated NS1 proteins could be good vaccine candidates.
PMID: 25934792 [PubMed - as supplied by publisher]
[h=1]The C-terminus of NS1 protein of influenza A/WSN/1933(H1N1) virus modulates antiviral responses in infected human macrophages and mice.[/h] Anastasina M[SUP]1[/SUP], Schepens B[SUP]2[/SUP], S?derholm S[SUP]3[/SUP], Nyman TA[SUP]4[/SUP], Matikainen S[SUP]3[/SUP], Saksela K[SUP]5[/SUP], Saelens X[SUP]2[/SUP], Kainov DE[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Non-structural protein NS1 of influenza A viruses interacts with cellular factors through its N-terminal RNA-binding, middle effector and C-terminal non-structured domains. NS1 attenuates antiviral responses in infected cells and thereby secures efficient virus replication. Some influenza strains express C-terminally truncated NS1 proteins due to nonsense mutations in NS1 gene. To understand the role of NS1 C-terminal region in regulation of antiviral responses, we engineered influenza viruses expressing C-terminally truncated NS1 proteins using A/WSN/33(H1N1) reverse-genetics and tested them in human macrophages and in mice. We showed that WSN virus expressing NS1 with a 28 amino acids deletion from its C-terminus is a more powerful inducer of antiviral responses than the virus expressing full-length NS1 or one with a 10 amino acid truncation of NS1. Thus, our finding suggests that the C-terminal region of NS1 is essential for regulation of antiviral responses. Moreover, viruses expressing truncated NS1 proteins could be good vaccine candidates.
PMID: 25934792 [PubMed - as supplied by publisher]