tetano
Editor, Senior Moderator
Cell Microbiol. 2012 Mar 14. doi: 10.1111/j.1462-5822.2012.01787.x. [Epub ahead of print]
The adaptor protein FHL2 enhances the cellular innate immune response to influenza A virus infection.
Nordhoff C, Hillesheim A, Walter B, Haasbach E, Planz O, Ehrhardt C, Ludwig S, Wixler V.
Source
Institute of Molecular Virology (IMV), Centre of Molecular Biology of Inflammation (ZMBE), University of Muenster, Von-Esmarch-Str. 56, D-48149 Muenster, Germany Interfaculty Institute for Cell Biology, Department of Immunology, Auf der Morgenstelle 15, 72076 Tuebingen, Germany.
Abstract
The innate immune response of influenza virus infected cells is predominantly mediated by type I interferon induced proteins. Expression of the interferon β (IFNβ) itself is initiated by accumulating viral RNA and is transmitted by different signaling cascades that feed into activation of the three transcriptional elements located in the IFNβ promoter, AP-1, IRF-3 and NF-κB. FHL2 (four-and-a-half LIM-domain protein 2) is an adaptor molecule that shuttles between membrane and nucleus regulating signaling cascades and gene transcription. Here we describe FHL2 as a novel regulator of influenza virus propagation. Using mouse FHL2 wild-type, knockout and rescued cells and human epithelial cells with different expression levels of FHL2 we showed that FHL2 decreases influenza A virus propagation by regulating the intrinsic cellular antiviral immune response. On virus infection FHL2 translocates into the nucleus, potentiating the IRF-3-dependent transcription of the IFNβ gene. ? 2012 Blackwell Publishing Ltd.
? 2012 Blackwell Publishing Ltd.
PMID:
22417706
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22417706
The adaptor protein FHL2 enhances the cellular innate immune response to influenza A virus infection.
Nordhoff C, Hillesheim A, Walter B, Haasbach E, Planz O, Ehrhardt C, Ludwig S, Wixler V.
Source
Institute of Molecular Virology (IMV), Centre of Molecular Biology of Inflammation (ZMBE), University of Muenster, Von-Esmarch-Str. 56, D-48149 Muenster, Germany Interfaculty Institute for Cell Biology, Department of Immunology, Auf der Morgenstelle 15, 72076 Tuebingen, Germany.
Abstract
The innate immune response of influenza virus infected cells is predominantly mediated by type I interferon induced proteins. Expression of the interferon β (IFNβ) itself is initiated by accumulating viral RNA and is transmitted by different signaling cascades that feed into activation of the three transcriptional elements located in the IFNβ promoter, AP-1, IRF-3 and NF-κB. FHL2 (four-and-a-half LIM-domain protein 2) is an adaptor molecule that shuttles between membrane and nucleus regulating signaling cascades and gene transcription. Here we describe FHL2 as a novel regulator of influenza virus propagation. Using mouse FHL2 wild-type, knockout and rescued cells and human epithelial cells with different expression levels of FHL2 we showed that FHL2 decreases influenza A virus propagation by regulating the intrinsic cellular antiviral immune response. On virus infection FHL2 translocates into the nucleus, potentiating the IRF-3-dependent transcription of the IFNβ gene. ? 2012 Blackwell Publishing Ltd.
? 2012 Blackwell Publishing Ltd.
PMID:
22417706
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22417706