tetano
Editor, Senior Moderator
Talanta
. 2024 Sep 25:281:126903.
doi: 10.1016/j.talanta.2024.126903. Online ahead of print. Enhanced performance of impedimetric immunosensors to detect SARS-CoV-2 with bare gold nanoparticles and graphene acetic acid
Rafael C Hensel[SUP] 1 [/SUP], Biagio Di Vizio[SUP] 2 [/SUP], Elsa M Materòn[SUP] 3 [/SUP], Flávio M Shimizu[SUP] 4 [/SUP], Monara Kaelle S C Angelim[SUP] 5 [/SUP], Gabriela F de Souza[SUP] 6 [/SUP], José L P Módena[SUP] 6 [/SUP], Pedro M M Moraes-Vieira[SUP] 5 [/SUP], Ricardo B de Azevedo[SUP] 7 [/SUP], Lucio Litti[SUP] 2 [/SUP], Stefano Agnoli[SUP] 2 [/SUP], Stefano Casalini[SUP] 2 [/SUP], Osvaldo N Oliveira Jr[SUP] 8 [/SUP]
Affiliations
Immunosensors based on electrical impedance spectroscopy allow for label-free, real-time detection of biologically relevant molecules and pathogens, without requiring electro-active materials. Here, we investigate the influence of bare gold nanoparticles (AuNPs), synthesized via laser ablation in solution, on the performance of an impedimetric immunosensor for detecting severe acute respiratory syndrome coronavirus (SARS-CoV-2). Graphene acetic acid (GAA) was used in the active layer for immobilizing anti-SARS-CoV-2 antibodies, owing to its high density of carboxylic groups. Immunosensors incorporating AuNPs exhibited superior performance compared to those relying solely on GAA, achieving a limit of detection (LoD) of 3 x 10[SUP]-20[/SUP] g/mL to detect the Spike Receptor Binding Domain (RBD) protein of SARS-CoV-2 and of 2 PFU/mL for inactivated virus. Moreover, these immunosensors presented high selectivity against the H1N1 influenza virus. We anticipate that this platform will be versatile and applicable in the early diagnosis of various diseases and viral infections, thereby facilitating Point-of-Care testing.
Keywords: Gold nanoparticles; Graphene acetic acid; Immunosensors; Impedance spectroscopy; SARS-CoV-2.
. 2024 Sep 25:281:126903.
doi: 10.1016/j.talanta.2024.126903. Online ahead of print. Enhanced performance of impedimetric immunosensors to detect SARS-CoV-2 with bare gold nanoparticles and graphene acetic acid
Rafael C Hensel[SUP] 1 [/SUP], Biagio Di Vizio[SUP] 2 [/SUP], Elsa M Materòn[SUP] 3 [/SUP], Flávio M Shimizu[SUP] 4 [/SUP], Monara Kaelle S C Angelim[SUP] 5 [/SUP], Gabriela F de Souza[SUP] 6 [/SUP], José L P Módena[SUP] 6 [/SUP], Pedro M M Moraes-Vieira[SUP] 5 [/SUP], Ricardo B de Azevedo[SUP] 7 [/SUP], Lucio Litti[SUP] 2 [/SUP], Stefano Agnoli[SUP] 2 [/SUP], Stefano Casalini[SUP] 2 [/SUP], Osvaldo N Oliveira Jr[SUP] 8 [/SUP]
Affiliations
- PMID: 39326119
- DOI: 10.1016/j.talanta.2024.126903
Immunosensors based on electrical impedance spectroscopy allow for label-free, real-time detection of biologically relevant molecules and pathogens, without requiring electro-active materials. Here, we investigate the influence of bare gold nanoparticles (AuNPs), synthesized via laser ablation in solution, on the performance of an impedimetric immunosensor for detecting severe acute respiratory syndrome coronavirus (SARS-CoV-2). Graphene acetic acid (GAA) was used in the active layer for immobilizing anti-SARS-CoV-2 antibodies, owing to its high density of carboxylic groups. Immunosensors incorporating AuNPs exhibited superior performance compared to those relying solely on GAA, achieving a limit of detection (LoD) of 3 x 10[SUP]-20[/SUP] g/mL to detect the Spike Receptor Binding Domain (RBD) protein of SARS-CoV-2 and of 2 PFU/mL for inactivated virus. Moreover, these immunosensors presented high selectivity against the H1N1 influenza virus. We anticipate that this platform will be versatile and applicable in the early diagnosis of various diseases and viral infections, thereby facilitating Point-of-Care testing.
Keywords: Gold nanoparticles; Graphene acetic acid; Immunosensors; Impedance spectroscopy; SARS-CoV-2.