tetano
Editor, Senior Moderator
J Vet Med Sci. 2018 Sep 11. doi: 10.1292/jvms.18-0291. [Epub ahead of print]
[h=1]System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila.[/h] Zhu C[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Liu X[SUP]4[/SUP], Chi H[SUP]4[/SUP], Chen C[SUP]1[/SUP], Chen Z[SUP]1[/SUP], Fu G[SUP]1[/SUP], Gong H[SUP]4[/SUP], Huang Y[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Tetrahymena is commonly used as an alternative eukaryotic system for efficiently expressing heterologous genes. In this study, we inserted the non-structural (NS) 1 gene of avian influenza virus (AIV) into the shuttle vector pD5H8 and transformed conjugating T. thermophila with the recombinant plasmid pD5H8-NS1 by particle bombardment. Positive transformants were selected with paromomycin. We demonstrated that the NS1 protein could be expressed steadily following induction with cadmium in this Tetrahymena system. An enzyme-linked immunosorbent assay detection method was preliminary established using the expressed protein as coating antigens for serodiagnosis. This is the first study in which a Tetrahymena expression system was employed for the expression of the AIV NS1 protein, and it provides a good basis for the development of differential diagnostic kits and vaccines for the prevention and control of avian influenza.
[h=4]KEYWORDS:[/h] NS1 gene; Tetrahymena; avian influenza; heterologous expression; virus
PMID: 30210069 DOI: 10.1292/jvms.18-0291
[h=1]System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila.[/h] Zhu C[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Liu X[SUP]4[/SUP], Chi H[SUP]4[/SUP], Chen C[SUP]1[/SUP], Chen Z[SUP]1[/SUP], Fu G[SUP]1[/SUP], Gong H[SUP]4[/SUP], Huang Y[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Tetrahymena is commonly used as an alternative eukaryotic system for efficiently expressing heterologous genes. In this study, we inserted the non-structural (NS) 1 gene of avian influenza virus (AIV) into the shuttle vector pD5H8 and transformed conjugating T. thermophila with the recombinant plasmid pD5H8-NS1 by particle bombardment. Positive transformants were selected with paromomycin. We demonstrated that the NS1 protein could be expressed steadily following induction with cadmium in this Tetrahymena system. An enzyme-linked immunosorbent assay detection method was preliminary established using the expressed protein as coating antigens for serodiagnosis. This is the first study in which a Tetrahymena expression system was employed for the expression of the AIV NS1 protein, and it provides a good basis for the development of differential diagnostic kits and vaccines for the prevention and control of avian influenza.
[h=4]KEYWORDS:[/h] NS1 gene; Tetrahymena; avian influenza; heterologous expression; virus
PMID: 30210069 DOI: 10.1292/jvms.18-0291