tetano
Editor, Senior Moderator
Viruses. 2018 Aug 9;10(8). pii: E419. doi: 10.3390/v10080419.
[h=1]Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production.[/h] Park HS[SUP]1,[/SUP][SUP]2[/SUP], Liu G[SUP]3,[/SUP][SUP]4[/SUP], Liu Q[SUP]5,[/SUP][SUP]6,[/SUP][SUP]7[/SUP], Zhou Y[SUP]8,[/SUP][SUP]9,[/SUP][SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Nucleotide-binding domain and leucine-rich repeat-containing protein 3 (NLRP3) inflammasome plays a pivotal role in modulating lung inflammation in response to the influenza A virus infection. We previously showed that the swine influenza virus (SIV) infection induced NLRP3 inflammasome-mediated IL-1β production in primary porcine alveolar macrophages (PAMs), and we were interested in examining the upstream signaling events that are involved in this process. Here, we report that the SIV-infection led to dynamin-related protein 1 (DRP1) phosphorylation at serine 579 and mitochondrial fission in PAMs. IL-1β production was dependent on the reactive oxygen species (ROS) production, and DRP1 phosphorylation resulted in the upregulation of the NLRP3 inflammasome. Furthermore, the requirement of the kinase activity of receptor-interacting protein kinase 1 (RIPK1) for the IL-1β production and RIPK1-DRP1 association suggested that RIPK1 is an upstream kinase for DRP1 phosphorylation. Our results reveal a critical role of the RIPK1/DRP1 signaling axis, whose activation leads to mitochondrial fission and ROS release, in modulating porcine NLRP3 inflammasome-mediated IL-1β production in SIV-infected PAMs.
[h=4]KEYWORDS:[/h] NLRP3 inflammasome; dynamin-related protein 1; interleukin-1 beta; mitochondrial fission; porcine alveolar macrophage; receptor-interacting protein kinase 1; swine influenza virus
PMID: 30096906 DOI: 10.3390/v10080419
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[h=1]Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production.[/h] Park HS[SUP]1,[/SUP][SUP]2[/SUP], Liu G[SUP]3,[/SUP][SUP]4[/SUP], Liu Q[SUP]5,[/SUP][SUP]6,[/SUP][SUP]7[/SUP], Zhou Y[SUP]8,[/SUP][SUP]9,[/SUP][SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Nucleotide-binding domain and leucine-rich repeat-containing protein 3 (NLRP3) inflammasome plays a pivotal role in modulating lung inflammation in response to the influenza A virus infection. We previously showed that the swine influenza virus (SIV) infection induced NLRP3 inflammasome-mediated IL-1β production in primary porcine alveolar macrophages (PAMs), and we were interested in examining the upstream signaling events that are involved in this process. Here, we report that the SIV-infection led to dynamin-related protein 1 (DRP1) phosphorylation at serine 579 and mitochondrial fission in PAMs. IL-1β production was dependent on the reactive oxygen species (ROS) production, and DRP1 phosphorylation resulted in the upregulation of the NLRP3 inflammasome. Furthermore, the requirement of the kinase activity of receptor-interacting protein kinase 1 (RIPK1) for the IL-1β production and RIPK1-DRP1 association suggested that RIPK1 is an upstream kinase for DRP1 phosphorylation. Our results reveal a critical role of the RIPK1/DRP1 signaling axis, whose activation leads to mitochondrial fission and ROS release, in modulating porcine NLRP3 inflammasome-mediated IL-1β production in SIV-infected PAMs.
[h=4]KEYWORDS:[/h] NLRP3 inflammasome; dynamin-related protein 1; interleukin-1 beta; mitochondrial fission; porcine alveolar macrophage; receptor-interacting protein kinase 1; swine influenza virus
PMID: 30096906 DOI: 10.3390/v10080419
Free full text