tetano
Editor, Senior Moderator
Transbound Emerg Dis. 2020 May 9. doi: 10.1111/tbed.13618. [Epub ahead of print]
Surveillance of influenza A virus subtype H5N1 in a live bird market in Yangon, Myanmar: 2017-2018.
Thurain K[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Mon PP[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Nasamran C[SUP]1,[/SUP][SUP]2[/SUP], Charoenkul K[SUP]1,[/SUP][SUP]2[/SUP], Boonyapisitsopa S[SUP]1,[/SUP][SUP]2[/SUP], Tun TN[SUP]3[/SUP], San YY[SUP]3[/SUP], Aye AM[SUP]3[/SUP], Amonsin A[SUP]1,[/SUP][SUP]2[/SUP].
Author information
Abstract
A survey of influenza A viruses (IAVs) in the Mingalar-Taung-Nyunt live bird market (MTN-LBM), Yangon, Myanmar, was conducted from December 2017 to December 2018. During the survey, 455 swab samples were collected from broilers, layers, backyard chickens and ducks from the MTN-LBM. Ninety-one pooled samples were screened for IAVs by real-time RT-PCR specific to the M gene. Positive pooled samples were individually retested for IAVs. In total, 2.63% of individual samples (12/455) were positive for IAVs. Out of 12 samples, 7 samples from layer chickens and the environment were identified as IAV subtype H5N1. In this study, 4 IAVs were successfully isolated and further characterized by whole-genome sequencing. Whole-genome sequence analysis revealed that the viruses were characterized as highly pathogenic avian influenza virus subtype H5N1 (HPAIV-H5N1) of clade 2.3.2.1c. Phylogenetic and genetic analyses showed that Myanmar HPAIV-H5N1 was closely related to HPAIV-H5N1 clade 2.3.2.1c isolated from China and Vietnam in 2014. Our results suggested that the live bird market in Myanmar represents a significant risk of HPAI-H5N1 transmission in poultry and humans. Moreover, HPAIV-H5N1 clade 2.3.2.1c is widely distributed in Southeast Asia including Myanmar.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Characterization; H5N1; Live bird market; Myanmar; Surveillance
PMID:32386461DOI:10.1111/tbed.13618
Surveillance of influenza A virus subtype H5N1 in a live bird market in Yangon, Myanmar: 2017-2018.
Thurain K[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Mon PP[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Nasamran C[SUP]1,[/SUP][SUP]2[/SUP], Charoenkul K[SUP]1,[/SUP][SUP]2[/SUP], Boonyapisitsopa S[SUP]1,[/SUP][SUP]2[/SUP], Tun TN[SUP]3[/SUP], San YY[SUP]3[/SUP], Aye AM[SUP]3[/SUP], Amonsin A[SUP]1,[/SUP][SUP]2[/SUP].
Author information
Abstract
A survey of influenza A viruses (IAVs) in the Mingalar-Taung-Nyunt live bird market (MTN-LBM), Yangon, Myanmar, was conducted from December 2017 to December 2018. During the survey, 455 swab samples were collected from broilers, layers, backyard chickens and ducks from the MTN-LBM. Ninety-one pooled samples were screened for IAVs by real-time RT-PCR specific to the M gene. Positive pooled samples were individually retested for IAVs. In total, 2.63% of individual samples (12/455) were positive for IAVs. Out of 12 samples, 7 samples from layer chickens and the environment were identified as IAV subtype H5N1. In this study, 4 IAVs were successfully isolated and further characterized by whole-genome sequencing. Whole-genome sequence analysis revealed that the viruses were characterized as highly pathogenic avian influenza virus subtype H5N1 (HPAIV-H5N1) of clade 2.3.2.1c. Phylogenetic and genetic analyses showed that Myanmar HPAIV-H5N1 was closely related to HPAIV-H5N1 clade 2.3.2.1c isolated from China and Vietnam in 2014. Our results suggested that the live bird market in Myanmar represents a significant risk of HPAI-H5N1 transmission in poultry and humans. Moreover, HPAIV-H5N1 clade 2.3.2.1c is widely distributed in Southeast Asia including Myanmar.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Characterization; H5N1; Live bird market; Myanmar; Surveillance
PMID:32386461DOI:10.1111/tbed.13618