tetano
Editor, Senior Moderator
Phytother Res. 2018 Sep 5. doi: 10.1002/ptr.6186. [Epub ahead of print]
[h=1]Suppression of influenza B virus replication by sakuranetin and mode of its action.[/h] Kwon DH[SUP]1[/SUP], Ji JH[SUP]2[/SUP], Yim SH[SUP]3[/SUP], Kim BS[SUP]4[/SUP], Choi HJ[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This study aimed to investigate in vitro the anti-influenza B/Lee/40 virus effect of sakuranetin and mode of its action. The sakuranetin exhibited potent antiviral activity against influenza B/Lee/40 virus, reducing the formation of a visible cytopathic effect, with a 50% inhibitory concentration (IC[SUB]50[/SUB] ) of 7.21 μg/ml and no cytotoxicity at a concentration of 100 μg/ml, and the derived therapeutic index (TI) was >13.87. Oseltamivir showed weak anti-influenza B/Lee/40 virus activity with IC[SUB]50[/SUB] of 80.74 μg/ml, 50% cytotoxicity concentration of >100 μg/ml, and TI of >1.24. Sakuranetin also showed effective inhibitory effects when added at the viral attachment, entry, and postentry steps. Moreover, sakuranetin effectively inactivated influenza B/Lee/40 virus infection in dose- and temperature-dependent manners. Sakuranetin indicated an inhibitory effect in viral RNA synthesis in the presence of 100 μg/ml of sakuranetin. Overall, this research revealed that sakuranetin could inhibit influenza B/Lee/40 virus replication and that sakuranetin may be involved in the virus attachment, entry, and postentry. Therefore, sakuranetin is a good candidate for a chemopreventive agent for influenza virus-related diseases.
[h=4]KEYWORDS:[/h] antiviral; influenza; mode; sakuranetin
PMID: 30187587 DOI: 10.1002/ptr.6186
[h=1]Suppression of influenza B virus replication by sakuranetin and mode of its action.[/h] Kwon DH[SUP]1[/SUP], Ji JH[SUP]2[/SUP], Yim SH[SUP]3[/SUP], Kim BS[SUP]4[/SUP], Choi HJ[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This study aimed to investigate in vitro the anti-influenza B/Lee/40 virus effect of sakuranetin and mode of its action. The sakuranetin exhibited potent antiviral activity against influenza B/Lee/40 virus, reducing the formation of a visible cytopathic effect, with a 50% inhibitory concentration (IC[SUB]50[/SUB] ) of 7.21 μg/ml and no cytotoxicity at a concentration of 100 μg/ml, and the derived therapeutic index (TI) was >13.87. Oseltamivir showed weak anti-influenza B/Lee/40 virus activity with IC[SUB]50[/SUB] of 80.74 μg/ml, 50% cytotoxicity concentration of >100 μg/ml, and TI of >1.24. Sakuranetin also showed effective inhibitory effects when added at the viral attachment, entry, and postentry steps. Moreover, sakuranetin effectively inactivated influenza B/Lee/40 virus infection in dose- and temperature-dependent manners. Sakuranetin indicated an inhibitory effect in viral RNA synthesis in the presence of 100 μg/ml of sakuranetin. Overall, this research revealed that sakuranetin could inhibit influenza B/Lee/40 virus replication and that sakuranetin may be involved in the virus attachment, entry, and postentry. Therefore, sakuranetin is a good candidate for a chemopreventive agent for influenza virus-related diseases.
[h=4]KEYWORDS:[/h] antiviral; influenza; mode; sakuranetin
PMID: 30187587 DOI: 10.1002/ptr.6186