tetano
Editor, Senior Moderator
Vet Microbiol. 2014 Jan 10. pii: S0378-1135(14)00017-0. doi: 10.1016/j.vetmic.2014.01.001. [Epub ahead of print]
Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection.
Park JK1, Lee DH1, Cho CH2, Yuk SS1, To EO1, Kwon JH1, Noh JY1, Kim BY1, Choi SW1, Shim BS2, Song MK2, Lee JB1, Park SY1, Choi IS1, Song CS3.
Author information
Abstract
Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza, Chicken, H9N2, M2e, Vaccine
PMID:
24472228
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24472228
Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection.
Park JK1, Lee DH1, Cho CH2, Yuk SS1, To EO1, Kwon JH1, Noh JY1, Kim BY1, Choi SW1, Shim BS2, Song MK2, Lee JB1, Park SY1, Choi IS1, Song CS3.
Author information
Abstract
Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza, Chicken, H9N2, M2e, Vaccine
PMID:
24472228
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24472228