Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device

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Methods Mol Biol. 2020;2063:17-25. doi: 10.1007/978-1-0716-0138-9_2. [h=1]Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device.[/h]
Larsen H[SUP]1[/SUP], Goecke NB[SUP]2[/SUP], Hjulsager CK[SUP]3[/SUP].
[h=3]Author information[/h] 1 National Veterinary Institute, Technical University of Denmark, Kongens Lyngby, Denmark. helela@vet.dtu.dk. 2 National Veterinary Institute, Technical University of Denmark, Kongens Lyngby, Denmark. 3 Statens Serum Institute, Artillerivej 5, Copenhagen, Denmark.

[h=3]Abstract[/h] Reverse transcription real-time PCR (RT-qPCR) is one of several techniques used to determine the presence and level of infectious veterinary pathogens in diagnostic laboratories. Here we describe how automation of PCR reactions using integrated fluidic circuits (IFCs), an IFC controller MX and a Biomark HD instrument allows for the testing of 48 field samples with swine influenza for up to 48 different subtypes simultaneously in nanoliter volumes.


[h=4]KEYWORDS:[/h] Biomark HD; Dynamic array (DA); Influenza A virus; Integrated fluidic circuits (IFCs); Microfluidics; Preamplification; Real-time PCR; Subtyping

PMID: 31667759 DOI: 10.1007/978-1-0716-0138-9_2