tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2015 Oct 7. pii: CVI.00443-15. [Epub ahead of print]
[h=1]Suboptimal humoral immune response against influenza A(H7N9) virus is related to its internal genes.[/h] Lee AC[SUP]1[/SUP], Zhu H[SUP]2[/SUP], Zhang AJ[SUP]3[/SUP], Li C[SUP]1[/SUP], Wang P[SUP]1[/SUP], Li C[SUP]1[/SUP], Chen H[SUP]3[/SUP], Hung IF[SUP]2[/SUP], To KK[SUP]3[/SUP], Yuen KY[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus A(H7N9) is associated with a high case fatality rate in humans. Multiple viral factors have been postulated to account for high virulence of the virus. It has been reported that patients with influenza A(H7N9) virus infection have relatively low titers of neutralizing antibody than those with seasonal influenza virus infections. In this study, we compared serum hemagglutination inhibition (HI) and microneutralization (MN) antibody titers of mice challenged with wild-type A(H7N9) viruses (H7N9[Anhui] and H7N9[Zhejiang]), an A(H1N1)pdm09 virus (pH1N1[2009]), and a recombinant A(H7N9) virus with PR8/H1N1 internal genes (rg-PR8-H7-N9). All mice infected by H7N9(Anhui) and H7N9(Zhejiang) developed serum HI antibody at 14 days post-infection (dpi), but no detectable MN antibody even at 28 dpi. A low level of neutralizing activity was detected in H7N9(Anhui)- and H7N9(Zhejiang)-infected mice using fluorescent foci MN assay, but convalescent sera obtained from H7N9(Anhui)-infected mice did not reduce mortality of na?ve mice after homologous virus challenge. Re-infection with homologous A(H7N9) virus induced higher HI and MN titers. In contrast, pH1N1(2009) virus infection induced robust HI and MN antibody responses even during the first infection. Moreover, rg-PR8-H7-N9 induced significantly higher HI and MN antibody titers. In conclusion, the internal genes of A(H7N9) virus can affect the humoral immune response against homologous viral surface proteins which may also contribute to the virulence of A(H7N9) virus.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26446420 [PubMed - as supplied by publisher]
[h=1]Suboptimal humoral immune response against influenza A(H7N9) virus is related to its internal genes.[/h] Lee AC[SUP]1[/SUP], Zhu H[SUP]2[/SUP], Zhang AJ[SUP]3[/SUP], Li C[SUP]1[/SUP], Wang P[SUP]1[/SUP], Li C[SUP]1[/SUP], Chen H[SUP]3[/SUP], Hung IF[SUP]2[/SUP], To KK[SUP]3[/SUP], Yuen KY[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus A(H7N9) is associated with a high case fatality rate in humans. Multiple viral factors have been postulated to account for high virulence of the virus. It has been reported that patients with influenza A(H7N9) virus infection have relatively low titers of neutralizing antibody than those with seasonal influenza virus infections. In this study, we compared serum hemagglutination inhibition (HI) and microneutralization (MN) antibody titers of mice challenged with wild-type A(H7N9) viruses (H7N9[Anhui] and H7N9[Zhejiang]), an A(H1N1)pdm09 virus (pH1N1[2009]), and a recombinant A(H7N9) virus with PR8/H1N1 internal genes (rg-PR8-H7-N9). All mice infected by H7N9(Anhui) and H7N9(Zhejiang) developed serum HI antibody at 14 days post-infection (dpi), but no detectable MN antibody even at 28 dpi. A low level of neutralizing activity was detected in H7N9(Anhui)- and H7N9(Zhejiang)-infected mice using fluorescent foci MN assay, but convalescent sera obtained from H7N9(Anhui)-infected mice did not reduce mortality of na?ve mice after homologous virus challenge. Re-infection with homologous A(H7N9) virus induced higher HI and MN titers. In contrast, pH1N1(2009) virus infection induced robust HI and MN antibody responses even during the first infection. Moreover, rg-PR8-H7-N9 induced significantly higher HI and MN antibody titers. In conclusion, the internal genes of A(H7N9) virus can affect the humoral immune response against homologous viral surface proteins which may also contribute to the virulence of A(H7N9) virus.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26446420 [PubMed - as supplied by publisher]