tetano
Editor, Senior Moderator
Bioorg Med Chem. 2019 Jul 19. pii: S0968-0896(19)31040-5. doi: 10.1016/j.bmc.2019.07.028. [Epub ahead of print]
[h=1]Structure-aided optimization of 3-O-β-chacotriosyl ursolic acid as novel H5N1 entry inhibitors with high selective index.[/h] Liao Y[SUP]1[/SUP], Chen L[SUP]2[/SUP], Li S[SUP]3[/SUP], Cui ZN[SUP]4[/SUP], Lei Z[SUP]5[/SUP], Li H[SUP]6[/SUP], Liu S[SUP]7[/SUP], Song G[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Currently, entry inhibitors contribute immensely in developing a new generation of anti-influenza virus drugs. Our earlier studies have identified that 3-O-β-chacotriosyl ursolic acid (1) could inhibit H5N1 pseudovirus by targeting hemagglutinin (HA). In the present study, a series of C-28 modified pentacyclic triterpene saponins via conjugation with a series of amide derivatives were synthesized and their antiviral activities against influenza A/Duck/Guangdong/99 virus (H5N1) in MDCK cells were evaluated. The SARs analysis of these compounds revealed that introduction of certain amide structures at the 17-COOH of ursolic acid could significantly enhance both their antiviral activity and selective index. This study indicated that the attachment of the methoxy group or Cl atom to the phenyl ring at the ortho- or para-position was crucial to improve inhibitory activity. Mechanism studies demonstrated that these title triterpenoids could bind tightly to the viral envelope HA to block the attachment of viruses to host cells, which was consistent with docking studies.
Copyright ? 2019 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] 3-O-β-chacotriosyl pentacyclic triterpenoids; H5N1 entry inhibitors; Structure-activity relationships
PMID: 31350154 DOI: 10.1016/j.bmc.2019.07.028
[h=1]Structure-aided optimization of 3-O-β-chacotriosyl ursolic acid as novel H5N1 entry inhibitors with high selective index.[/h] Liao Y[SUP]1[/SUP], Chen L[SUP]2[/SUP], Li S[SUP]3[/SUP], Cui ZN[SUP]4[/SUP], Lei Z[SUP]5[/SUP], Li H[SUP]6[/SUP], Liu S[SUP]7[/SUP], Song G[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Currently, entry inhibitors contribute immensely in developing a new generation of anti-influenza virus drugs. Our earlier studies have identified that 3-O-β-chacotriosyl ursolic acid (1) could inhibit H5N1 pseudovirus by targeting hemagglutinin (HA). In the present study, a series of C-28 modified pentacyclic triterpene saponins via conjugation with a series of amide derivatives were synthesized and their antiviral activities against influenza A/Duck/Guangdong/99 virus (H5N1) in MDCK cells were evaluated. The SARs analysis of these compounds revealed that introduction of certain amide structures at the 17-COOH of ursolic acid could significantly enhance both their antiviral activity and selective index. This study indicated that the attachment of the methoxy group or Cl atom to the phenyl ring at the ortho- or para-position was crucial to improve inhibitory activity. Mechanism studies demonstrated that these title triterpenoids could bind tightly to the viral envelope HA to block the attachment of viruses to host cells, which was consistent with docking studies.
Copyright ? 2019 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] 3-O-β-chacotriosyl pentacyclic triterpenoids; H5N1 entry inhibitors; Structure-activity relationships
PMID: 31350154 DOI: 10.1016/j.bmc.2019.07.028