tetano
Editor, Senior Moderator
J Virol Methods. 2017 Oct 4;251:7-14. doi: 10.1016/j.jviromet.2017.10.003. [Epub ahead of print]
[h=1]Strategies to obtain multiple recombinant modified vaccinia Ankara vectors. Applications to influenza vaccines.[/h] Barbieri A[SUP]1[/SUP], Panigada M[SUP]2[/SUP], Soprana E[SUP]2[/SUP], Di Mario G[SUP]3[/SUP], Gubinelli F[SUP]2[/SUP], Bernasconi V[SUP]2[/SUP], Recagni M[SUP]2[/SUP], Donatelli I[SUP]3[/SUP], Castrucci MR[SUP]3[/SUP], Siccardi AG[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] As a vaccination vector, MVA has been widely investigated both in animal models and humans. The construction of recombinant MVA (rMVA) relies on homologous recombination between an acceptor virus and a donor plasmid in infected/transfected permissive cells. Our construction strategy "Red-to-Green gene swapping" - based on the exchange of two fluorescent markers within the flanking regions of MVA deletion ΔIII, coupled to fluorescence activated cell sorting - is here extended to a second insertion site, within the flanking regions of MVA deletion ΔVI. Exploiting this strategy, both double and triple rMVA were constructed, expressing as transgenes the influenza A proteins HA, NP, M1, and PB1. Upon validation of the harbored transgenes co-expression, double and triple recombinants rMVA(ΔIII)-NP-P2A-M1 and rMVA(ΔIII)-NP-P2A-M1-(ΔVI)-PB1 were assayed for in vivo immunogenicity and protection against lethal challenge. In vivo responses were identical to those obtained with the reported combinations of single recombinants, supporting the feasibility and reliability of the present improvement and the extension of Red-to-Green gene swapping to insertion sites other than ΔIII.
Copyright ? 2017. Published by Elsevier B.V.
PMID: 28987424 DOI: 10.1016/j.jviromet.2017.10.003
[h=1]Strategies to obtain multiple recombinant modified vaccinia Ankara vectors. Applications to influenza vaccines.[/h] Barbieri A[SUP]1[/SUP], Panigada M[SUP]2[/SUP], Soprana E[SUP]2[/SUP], Di Mario G[SUP]3[/SUP], Gubinelli F[SUP]2[/SUP], Bernasconi V[SUP]2[/SUP], Recagni M[SUP]2[/SUP], Donatelli I[SUP]3[/SUP], Castrucci MR[SUP]3[/SUP], Siccardi AG[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] As a vaccination vector, MVA has been widely investigated both in animal models and humans. The construction of recombinant MVA (rMVA) relies on homologous recombination between an acceptor virus and a donor plasmid in infected/transfected permissive cells. Our construction strategy "Red-to-Green gene swapping" - based on the exchange of two fluorescent markers within the flanking regions of MVA deletion ΔIII, coupled to fluorescence activated cell sorting - is here extended to a second insertion site, within the flanking regions of MVA deletion ΔVI. Exploiting this strategy, both double and triple rMVA were constructed, expressing as transgenes the influenza A proteins HA, NP, M1, and PB1. Upon validation of the harbored transgenes co-expression, double and triple recombinants rMVA(ΔIII)-NP-P2A-M1 and rMVA(ΔIII)-NP-P2A-M1-(ΔVI)-PB1 were assayed for in vivo immunogenicity and protection against lethal challenge. In vivo responses were identical to those obtained with the reported combinations of single recombinants, supporting the feasibility and reliability of the present improvement and the extension of Red-to-Green gene swapping to insertion sites other than ΔIII.
Copyright ? 2017. Published by Elsevier B.V.
PMID: 28987424 DOI: 10.1016/j.jviromet.2017.10.003